CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection
dc.contributor.author | Vaněčková, Tereza | cs |
dc.contributor.author | Bezděková, Jaroslava | cs |
dc.contributor.author | Kuchynka, Michaela | cs |
dc.contributor.author | Vlčnovská, Marcela | cs |
dc.contributor.author | Novotná, Veronika | cs |
dc.contributor.author | Neuman, Jan | cs |
dc.contributor.author | Štossová, Aneta | cs |
dc.contributor.author | Kanický, Viktor | cs |
dc.contributor.author | Adam, Vojtěch | cs |
dc.contributor.author | Vaculovičová, Markéta | cs |
dc.contributor.author | Vaculovič, Tomáš | cs |
dc.coverage.issue | 1 | cs |
dc.coverage.volume | 9 | cs |
dc.date.issued | 2019-08-14 | cs |
dc.description.abstract | For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-anti body and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample. | en |
dc.format | text | cs |
dc.format.extent | 1-9 | cs |
dc.format.mimetype | application/pdf | cs |
dc.identifier.citation | Scientific Reports. 2019, vol. 9, issue 1, p. 1-9. | en |
dc.identifier.doi | 10.1038/s41598-019-48290-2 | cs |
dc.identifier.issn | 2045-2322 | cs |
dc.identifier.orcid | 0000-0002-1686-4423 | cs |
dc.identifier.orcid | 0000-0003-0408-2771 | cs |
dc.identifier.orcid | 0000-0002-8527-286X | cs |
dc.identifier.orcid | 0000-0002-6771-1304 | cs |
dc.identifier.other | 158577 | cs |
dc.identifier.researcherid | ABE-5364-2021 | cs |
dc.identifier.researcherid | AAC-3839-2019 | cs |
dc.identifier.researcherid | D-7686-2012 | cs |
dc.identifier.researcherid | E-5583-2016 | cs |
dc.identifier.scopus | 53464444600 | cs |
dc.identifier.scopus | 57195612235 | cs |
dc.identifier.uri | http://hdl.handle.net/11012/180684 | |
dc.language.iso | en | cs |
dc.publisher | Springer Nature | cs |
dc.relation.ispartof | Scientific Reports | cs |
dc.relation.uri | http://link.springer.com/article/10.1038/s41598-019-48290-2 | cs |
dc.rights | Creative Commons Attribution 4.0 International | cs |
dc.rights.access | openAccess | cs |
dc.rights.sherpa | http://www.sherpa.ac.uk/romeo/issn/2045-2322/ | cs |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | cs |
dc.subject | CdS quantum dots (QDs) | en |
dc.subject | molecularly imprinted polymer (MIP) | en |
dc.subject | laser ablation ICP-MS | en |
dc.title | CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection | en |
dc.type.driver | article | en |
dc.type.status | Peer-reviewed | en |
dc.type.version | publishedVersion | en |
sync.item.dbid | VAV-158577 | en |
sync.item.dbtype | VAV | en |
sync.item.insts | 2025.02.03 15:49:52 | en |
sync.item.modts | 2025.01.17 19:36:19 | en |
thesis.grantor | Vysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástroje | cs |
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