Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells

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dc.contributor.authorOliver, Carolinacs
dc.contributor.authorIbaez, Raúl Rosalescs
dc.contributor.authorFlores-Merino, Miriamcs
dc.contributor.authorVojtová, Lucycs
dc.contributor.authorŠalplachta, Jakubcs
dc.contributor.authorČelko, Ladislavcs
dc.contributor.authorKaiser, Jozefcs
dc.contributor.authorMontufar Jimenez, Edgar Benjamincs
dc.coverage.issue1cs
dc.coverage.volume14cs
dc.date.issued2021-01-05cs
dc.description.abstractThis work shows the synthesis of a polyvinylpyrrolidone (PVP) hydrogel by heat-activated polymerization and explores the production of hydrogels with an open porous network by lyophilisation to allow the three-dimensional culture of human oral mucosa stem cells (hOMSCs). The swollen hydrogel showed a storage modulus similar to oral mucosa and elastic solid rheological behaviour without sol transition. A comprehensive characterization of porosity by scanning electron microscopy, mercury intrusion porosimetry and nano-computed tomography (with spatial resolution below 1 mu m) showed that lyophilisation resulted in the heterogeneous incorporation of closed oval-like pores in the hydrogel with broad size distribution (5 to 180 mu m, d(50) = 65 mu m). Human oral mucosa biopsies were used to isolate hOMSCs, expressing typical markers of mesenchymal stem cells in more than 95% of the cell population. Direct contact cytotoxicity assay demonstrated that PVP hydrogel have no negative effect on cell metabolic activity, allowing the culture of hOMSCs with normal fusiform morphology. Pore connectivity should be improved in future to allow cell growth in the bulk of the PVP hydrogel.en
dc.formattextcs
dc.format.extent1-14cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMaterials . 2021, vol. 14, issue 1, p. 1-14.en
dc.identifier.doi10.3390/ma14010227cs
dc.identifier.issn1996-1944cs
dc.identifier.orcid0000-0001-5281-7045cs
dc.identifier.orcid0000-0002-0149-7843cs
dc.identifier.orcid0000-0003-0264-3483cs
dc.identifier.orcid0000-0002-7397-125Xcs
dc.identifier.orcid0000-0002-8122-4000cs
dc.identifier.other168361cs
dc.identifier.researcheridD-7762-2012cs
dc.identifier.researcheridR-1104-2018cs
dc.identifier.researcheridD-6870-2012cs
dc.identifier.researcheridD-6800-2012cs
dc.identifier.researcheridF-8040-2016cs
dc.identifier.scopus12039667200cs
dc.identifier.scopus25621022900cs
dc.identifier.scopus7402184758cs
dc.identifier.scopus23397943300cs
dc.identifier.urihttp://hdl.handle.net/11012/195922
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMaterialscs
dc.relation.urihttps://www.mdpi.com/1996-1944/14/1/227cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1996-1944/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectpolyvinylpyrrolidoneen
dc.subjecthydrogelen
dc.subjectlyophilisationen
dc.subjectnano-computed tomographyen
dc.subjectporosityen
dc.subjectoral mucosaen
dc.subjectstem cellen
dc.titleLyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cellsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-168361en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:51:00en
sync.item.modts2025.01.17 16:39:40en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé instrumentace a metody pro charakterizace materiálůcs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé biomateriálycs
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