Antibacterial activity of therapeutic agent-immobilized nanostructured TiCaPCON films against antibiotic-sensitive and antibiotic-resistant Escherichia coli strains

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dc.contributor.authorPermyakova, Elizavetacs
dc.contributor.authorKiryukhantsev-Korneev, Philipcs
dc.contributor.authorPonomarev, Viktor A.cs
dc.contributor.authorSheveyko, A.N.cs
dc.contributor.authorDobrynin, Sergey A.cs
dc.contributor.authorPolčák, Josefcs
dc.contributor.authorSlukin, Pavel V.cs
dc.contributor.authorIgnatov, Sergei G.cs
dc.contributor.authorManakhov, Antoncs
dc.contributor.authorKulinich, Sergei A.cs
dc.contributor.authorShtansky, Dmitry V.cs
dc.coverage.issue1cs
dc.coverage.volume405cs
dc.date.accessioned2021-08-10T10:53:15Z
dc.date.available2021-08-10T10:53:15Z
dc.date.issued2021-01-15cs
dc.description.abstractThe development of flexible and low-cost methods of surface functionalization to fight infection at the early stage is an urgent scientific task. Herein, polymerization in low-pressure plasma rich in COOH species and carbodii-mide chemistry methods were utilized to immobilize four different therapeutic agents (antibiotic (gentamicin), antimicrobial peptide (indolicidin), anti-adhesive molecules (heparin) and nitroxide radicals (2,2,5,5-tetramethyl-3-carboxyl-pyrrolidine-1-oxyl)) on the surface of nanostructured biocompatible TiCaPCON films to impart antibacterial characteristics. The polymers deposited from COOH-rich plasma showed decent stability in phosphate-buffered saline solution and were successfully used for the immobilization of different therapeutic agents via ionic or covalent bond. The bactericide attachment was proved by FTIR spectroscopy and XPS analysis. All samples with grafted therapeutic agents were hydrophilic with water contact angle values in the range of 26-56 degrees. Bactericide release tests indicated the maximum concentration of therapeutic agents in the case of ionic immobilization. In case of covalent immobilization, fast initial release observed over 24 h was followed by slower leaching in the next 24 h (indolicidin), 48 h (heparin), and 96 h (gentamicin). The pH-sensitive COOH plasma polymer degradation and gentamicin release were demonstrated. The bactericide-linked films showed noticeable reduction of the antibiotic-sensitive E. coli U20 strain and, except indolicidin-immobilized samples, effectively inhibited growth of the antibiotic-resistant E. coli K261 strain at their initial concentration of 10(4) CFU/mL. The films with nitroxide radicals not only exhibited the highest antibacterial activity against E. coli K261 cells (100% after 8 h), but also prevented the biofilm formation.en
dc.description.embargo2022-10-24cs
dc.formattextcs
dc.format.extent126538-1-126538-15cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationSurface and Coatings Technology. 2021, vol. 405, issue 1, p. 126538-1-126538-15.en
dc.identifier.doi10.1016/j.surfcoat.2020.126538cs
dc.identifier.issn0257-8972cs
dc.identifier.other172147cs
dc.identifier.urihttp://hdl.handle.net/11012/200925
dc.language.isoencs
dc.publisherElseviercs
dc.relation.ispartofSurface and Coatings Technologycs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0257897220312081cs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0257-8972/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectBiocompatible filmsen
dc.subjectPlasma polymerizationen
dc.subjectSurface immobilizationen
dc.subjectBactericide releaseen
dc.subjectAntibacterial activityen
dc.subjectTherapeutic agentsen
dc.titleAntibacterial activity of therapeutic agent-immobilized nanostructured TiCaPCON films against antibiotic-sensitive and antibiotic-resistant Escherichia coli strainsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionacceptedVersionen
sync.item.dbidVAV-172147en
sync.item.dbtypeVAVen
sync.item.insts2022.10.24 01:02:53en
sync.item.modts2022.10.24 00:17:48en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Sdílená laboratoř RP1cs
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav fyzikálního inženýrstvícs
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