Tuning the surface coating of IONs toward efficient sonochemical tethering and sustained liberation of topoisomerase II poisons

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dc.contributor.authorMichálková, Hanacs
dc.contributor.authorStrmiska, Vladislavcs
dc.contributor.authorKudr, Jiřícs
dc.contributor.authorŠkubalová, Zuzanacs
dc.contributor.authorTesařová, Barboracs
dc.contributor.authorŠvec, Pavelcs
dc.contributor.authorRichtera, Lukášcs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorHeger, Zbyněkcs
dc.coverage.issue1cs
dc.coverage.volume14cs
dc.date.issued2019-09-17cs
dc.description.abstractBackground: Iron oxide nanoparticles (IONs) have been increasingly utilized in a wide spectrum of biomedical applications. Surface coatings of IONs can bestow a number of exceptional properties, including enhanced stability of IONs, increased loading of drugs or their controlled release. Methods: Using two-step sonochemical protocol, IONs were surface-coated with polyoxyethylene stearate, polyvinylpyrrolidone or chitosan for a loading of two distinct topo II poisons (doxorubicin and ellipticine). The cytotoxic behavior was tested in vitro against breast cancer (MDA-MB-231) and healthy epithelial cells (HEK-293 and HBL-100). In addition, biocompatibility studies (hemotoxicity, protein corona formation, binding of third complement component) were performed. Results: Notably, despite surface-coated IONs exhibited only negligible cytotoxicity, upon tethering with topo II poisons, synergistic or additional enhancement of cytotoxicity was found in MDA-MB-231 cells. Pronounced anti-migratory activity, DNA fragmentation, decrease in expression of procaspase-3 and enhancement of p53 expression were further identified upon exposure to surface-coated IONs with tethered doxorubicin and ellipticine. Moreover, surface-coated IONs nanoformulations of topo II poisons exhibited exceptional stability in human plasma with no protein corona and complement 3 binding, and only a mild induction of hemolysis in human red blood cells. Conclusion: The results imply a high potential of an efficient ultrasound-mediated surface functionalization of IONs as delivery vehicles to improve therapeutic efficiency of topo II poisons.en
dc.formattextcs
dc.format.extent7609-7624cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Nanomedicine. 2019, vol. 14, issue 1, p. 7609-7624.en
dc.identifier.doi10.2147/IJN.S208810cs
dc.identifier.issn1178-2013cs
dc.identifier.orcid0000-0002-7036-1640cs
dc.identifier.orcid0000-0002-8288-3999cs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.orcid0000-0002-3915-7270cs
dc.identifier.other160088cs
dc.identifier.researcheridH-2870-2018cs
dc.identifier.researcheridN-9991-2014cs
dc.identifier.researcheridE11072012cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.researcheridD-1973-2013cs
dc.identifier.scopus12040049600cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/181092
dc.language.isoencs
dc.publisherDove Medical Presscs
dc.relation.ispartofInternational Journal of Nanomedicinecs
dc.relation.urihttps://www.dovepress.com/tuning-the-surface-coating-of-ions-toward-efficient-sonochemical-tethe-peer-reviewed-article-IJNcs
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unportedcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1178-2013/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/cs
dc.subjectdoxorubicinen
dc.subjectellipticineen
dc.subjectiron oxideen
dc.subjectnanoparticlesen
dc.subjectrelease kineticsen
dc.titleTuning the surface coating of IONs toward efficient sonochemical tethering and sustained liberation of topoisomerase II poisonsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-160088en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:49:53en
sync.item.modts2025.01.17 15:22:58en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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