Nanoparticle-based FRET pair selection for investigation of metallothionein dimerization

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dc.contributor.authorPavelicová, Kristýnacs
dc.contributor.authorPompeiano Vaníčková, Luciecs
dc.contributor.authorVlčnovská, Marcelacs
dc.contributor.authorVaculovičová, Markétacs
dc.date.accessioned2021-05-04T10:54:19Z
dc.date.available2021-05-04T10:54:19Z
dc.date.issued2020-06-10cs
dc.description.abstractMetalloproteins (MTs) are one of the most diverse classes of proteins, with the intrinsic metal atoms providing a catalytic, regulatory and structural role crucial to protein functioning. When stored under aerobic conditions, MTs might form dimers (as well as higher oligomers) that play an essential role as mediators in oxidoreduction signalling pathways. In this work, we explore the non-oxidative dimerization process characterized by metal (Cd) bridging of MT monomers by Förster resonance energy transfer. The formation of MT dimers/oligomers was investigated by applying capillary electrophoresis and matrix-assisted laser desorption/ionization with mass spectrometric detection.en
dc.formattextcs
dc.format.extent406-410cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationNANOCON 2019 CONFERENCE PROCEEDINGS. 2020, p. 406-410.en
dc.identifier.doi10.37904/nanocon.2019.8566cs
dc.identifier.isbn978-80-87294-95-6cs
dc.identifier.other164256cs
dc.identifier.urihttp://hdl.handle.net/11012/196669
dc.language.isoencs
dc.publisherTangercs
dc.relation.ispartofNANOCON 2019 CONFERENCE PROCEEDINGScs
dc.relation.urihttps://doi.org/10.37904/nanocon.2019.8566cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectMetallothioneinen
dc.subjectdimerizationen
dc.subjectcapillary electrophoresisen
dc.subjectFörster resonance energy transferen
dc.titleNanoparticle-based FRET pair selection for investigation of metallothionein dimerizationen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-164256en
sync.item.dbtypeVAVen
sync.item.insts2022.01.11 16:55:47en
sync.item.modts2022.01.11 16:15:22en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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