The Isolation of DNA by Polycharged Magnetic Particles: An Analysis of the Interaction by Zeta Potential and Particle Size

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dc.contributor.authorHaddad, Yazan Abdulmajeed Eyadhcs
dc.contributor.authorXhaxhiu, Kledics
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorHynek, Davidcs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorAdam, Vojtěchcs
dc.coverage.issue4cs
dc.coverage.volume17cs
dc.date.issued2016-04-20cs
dc.description.abstractMagnetic isolation of biological targets is in major demand in the biotechnology industry today. This study considers the interaction of four surface-modified magnetic micro- and nanoparticles with selected DNA fragments. Different surface modifications of nanomaghemite precursors were investigated: MAN37 (silica-coated), MAN127 (polyvinylpyrrolidone-coated), MAN158 (phosphate-coated), and MAN164 (tripolyphosphate-coated). All particles were positive polycharged agglomerated monodispersed systems. Mean particle sizes were 0.48, 2.97, 2.93, and 3.67 m for MAN37, MAN127, MAN164, and MAN158, respectively. DNA fragments exhibited negative zeta potential of 0.22 mV under binding conditions (high ionic strength, low pH, and dehydration). A decrease in zeta potential of particles upon exposure to DNA was observed with exception of MAN158 particles. The measured particle size of MAN164 particles increased by nearly twofold upon exposure to DNA. Quantitative PCR isolation of DNA with a high retrieval rate was observed by magnetic particles MAN127 and MAN164. Interaction between polycharged magnetic particles and DNA is mediated by various binding mechanisms such as hydrophobic and electrostatic interactions. Future development of DNA isolation technology requires an understanding of the physical and biochemical conditions of this process.en
dc.description.abstractMagnetic isolation of biological targets is in major demand in the biotechnology industry today. This study considers the interaction of four surface-modified magnetic micro- and nanoparticles with selected DNA fragments. Different surface modifications of nanomaghemite precursors were investigated: MAN37 (silica-coated), MAN127 (polyvinylpyrrolidone-coated), MAN158 (phosphate-coated), and MAN164 (tripolyphosphate-coated). All particles were positive polycharged agglomerated monodispersed systems. Mean particle sizes were 0.48, 2.97, 2.93, and 3.67 m for MAN37, MAN127, MAN164, and MAN158, respectively. DNA fragments exhibited negative zeta potential of 0.22 mV under binding conditions (high ionic strength, low pH, and dehydration). A decrease in zeta potential of particles upon exposure to DNA was observed with exception of MAN158 particles. The measured particle size of MAN164 particles increased by nearly twofold upon exposure to DNA. Quantitative PCR isolation of DNA with a high retrieval rate was observed by magnetic particles MAN127 and MAN164. Interaction between polycharged magnetic particles and DNA is mediated by various binding mechanisms such as hydrophobic and electrostatic interactions. Future development of DNA isolation technology requires an understanding of the physical and biochemical conditions of this process.en
dc.formattextcs
dc.format.extent550-1-550-11cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2016, vol. 17, issue 4, p. 550-1-550-11.en
dc.identifier.doi10.3390/ijms17040550cs
dc.identifier.issn1661-6596cs
dc.identifier.orcid0000-0002-7844-4336cs
dc.identifier.orcid0000-0003-4216-9544cs
dc.identifier.orcid0000-0002-7318-6470cs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.other126823cs
dc.identifier.researcheridJ-2985-2015cs
dc.identifier.researcheridE-5711-2012cs
dc.identifier.researcheridE-5702-2012cs
dc.identifier.researcheridE11072012cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.scopus36070488100cs
dc.identifier.scopus6603604023cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/63814
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofINTERNATIONAL JOURNAL OF MOLECULAR SCIENCEScs
dc.relation.urihttp://www.mdpi.com/1422-0067/17/4/550cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1661-6596/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectmagneticen
dc.subjectnanoparticlesen
dc.subjectDNA isolationen
dc.subjectzeta potentialen
dc.subjectparticle sizeen
dc.subjectmagnetic
dc.subjectnanoparticles
dc.subjectDNA isolation
dc.subjectzeta potential
dc.subjectparticle size
dc.titleThe Isolation of DNA by Polycharged Magnetic Particles: An Analysis of the Interaction by Zeta Potential and Particle Sizeen
dc.title.alternativeThe Isolation of DNA by Polycharged Magnetic Particles: An Analysis of the Interaction by Zeta Potential and Particle Sizeen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-126823en
sync.item.dbtypeVAVen
sync.item.insts2025.10.14 15:17:08en
sync.item.modts2025.10.14 10:14:02en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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