A quantitative analysis of 3D-cell distribution in regenerating muscle-skeletal system with synchrotron X-ray computed microtomography

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dc.contributor.authorKaiser, Markétacs
dc.contributor.authorKalasová, Dominikacs
dc.contributor.authorZikmund, Tomášcs
dc.contributor.authorNovotná, Mariecs
dc.contributor.authorKaiser, Jozefcs
dc.coverage.issue1cs
dc.coverage.volume8cs
dc.date.issued2018-09-20cs
dc.description.abstractOne of the greatest enigmas of modern biology is how the geometry of muscular and skeletal structures are created and how their development is controlled during growth and regeneration. Scaling and shaping of vertebrate muscles and skeletal elements has always been enigmatic and required an advanced technical level in order to analyse the cell distribution in 3D. In this work, synchrotron X-ray computed microtomography (CT) and chemical contrasting has been exploited for a quantitative analysis of the 3D-cell distribution in tissues of a developing salamander (Pleurodeles waltl) limb – a key model organism for vertebrate regeneration studies. We mapped the limb muscles, their size and shape as well as the number and density of cells within the extracellular matrix of the developing cartilage. By using tomographic approach, we explored the polarity of the cells in 3D, in relation to the structure of developing joints. We found that the polarity of chondrocytes correlates with the planes in joint surfaces and also changes along the length of the cartilaginous elements. Our approach generates data for the precise computer simulations of muscle-skeletal regeneration using cell dynamics models, which is necessary for the understanding how anisotropic growth results in the precise shapes of skeletal structures.en
dc.formattextcs
dc.format.extent1-13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationScientific Reports. 2018, vol. 8, issue 1, p. 1-13.en
dc.identifier.doi10.1038/s41598-018-32459-2cs
dc.identifier.issn2045-2322cs
dc.identifier.orcid0000-0002-5200-7365cs
dc.identifier.orcid0000-0003-3559-9562cs
dc.identifier.orcid0000-0003-2948-5198cs
dc.identifier.orcid0000-0002-7397-125Xcs
dc.identifier.other150099cs
dc.identifier.researcheridV-3696-2018cs
dc.identifier.researcheridG-9491-2014cs
dc.identifier.researcheridD-6800-2012cs
dc.identifier.scopus7402184758cs
dc.identifier.urihttp://hdl.handle.net/11012/85329
dc.language.isoencs
dc.publisherSpringer Naturecs
dc.relation.ispartofScientific Reportscs
dc.relation.urihttp://link.springer.com/article/10.1038/s41598-018-32459-2cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2045-2322/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectCTen
dc.subjecttomographyen
dc.subject3Den
dc.subjectcellen
dc.subjectsalamanderen
dc.titleA quantitative analysis of 3D-cell distribution in regenerating muscle-skeletal system with synchrotron X-ray computed microtomographyen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-150099en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 16:10:53en
sync.item.modts2025.01.17 15:22:08en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé instrumentace a metody pro charakterizace materiálůcs
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Pokročilé instrumentace a metody pro charakterizace materiálů