Denitrification assays for testing effects of xenobiotics on aquatic denitrification and their degradation in aquatic environments

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dc.contributor.authorPastorek Pániková, Kristínacs
dc.contributor.authorWeigelhofer, Gabrielecs
dc.contributor.authorBílková, Zuzanacs
dc.contributor.authorMalá, Jitkacs
dc.coverage.issue14cs
dc.coverage.volume15cs
dc.date.issued2023-07-11cs
dc.description.abstractWe developed, tested, and optimized two laboratory denitrification assays for both managers and scientists to assess the effects of xenobiotics on the denitrification process over 7 days (short batch assay, SBA) and 28 days (long semi-continuous assay, LSA). The assays facilitate (1) measuring the efficiency of nitrate removal under the influence of xenobiotics, (2) determining the removal of the tested xenobiotics via adsorption or biotic decomposition, and (3) testing the influencing parameters for optimizing the denitrification process. The adsorption of the xenobiotics was assessed by inhibiting all biological processes through the addition of HgCl2. Our tests demonstrate that the ratio of the initial nitrate concentration to the amount of bioavailable organic matter provided is essential to avoid organic carbon or nitrate limitation. While a pH less than 7 resulted in decreased denitrification, a pH more than 8 let to nitrite accumulation, indicating incomplete denitrification. Over durations of more than a week, weekly replenishments of the nitrate and HgCl2 and weekly purging with argon gas to reduce the oxygen concentrations are needed. The assays provide information about the accumulation of xenobiotics in the bioreactors that is necessary for the environmentally friendly treatment of the bioreactor fillings and provide insight into the potential of the bioreactors to remove pesticides from polluted water resources.en
dc.formattextcs
dc.format.extent13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationWater. 2023, vol. 15, issue 14, 13 p.en
dc.identifier.doi10.3390/w15142536cs
dc.identifier.issn2073-4441cs
dc.identifier.orcid0000-0002-8034-6495cs
dc.identifier.orcid0000-0002-1399-0694cs
dc.identifier.other184193cs
dc.identifier.researcheridN-6575-2018cs
dc.identifier.scopus35490786100cs
dc.identifier.urihttp://hdl.handle.net/11012/244999
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofWatercs
dc.relation.urihttps://www.mdpi.com/2073-4441/15/14/2536cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2073-4441/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectdenitrificationen
dc.subjectxenobioticsen
dc.subjectbatch assaysen
dc.subjectmethod testingen
dc.subjectremoval efficiencyen
dc.subjectmetolachloren
dc.titleDenitrification assays for testing effects of xenobiotics on aquatic denitrification and their degradation in aquatic environmentsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-184193en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:44:24en
sync.item.modts2025.01.17 16:50:11en
thesis.grantorVysoké učení technické v Brně. Fakulta stavební. Ústav chemiecs
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