The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed Metschnikowia sp. Yeasts
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Date
2021-01-29
Authors
Němcová, Andrea
Gonová, Dominika
Samek, Ota
Sipiczki, Matthias
Breierova, Emilia
Márová, Ivana
0000-0002-1164-1837Advisor
Referee
Mark
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MDPI
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Abstract
Raman spectroscopy is a universal method designed for the analysis of a wide range of physical, chemical and biological systems or various surfaces. This technique is suitable to monitor various components of cells, tissues or microorganisms. The advantages include very fast non-contact and non-destructive analysis and no or minimal need for sample treatment. The yeasts Metschnikowia can be considered as industrially usable producers of pulcherrimin or single-cell lipids, depending on cultivation conditions and external stress. In the present study, Raman spectroscopy was used as an effective tool to identify both pulcherrimin and lipids in single yeast cells.
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Peer-reviewed
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en