A chemometric-assisted voltammetric analysis of free and Zn(II)-loaded metallothionein-3 states

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dc.contributor.authorPeris-Díaz, Manuel Davidcs
dc.contributor.authorRichtera, Lukášcs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorKrężel, Arturcs
dc.contributor.authorAdam, Vojtěchcs
dc.coverage.issue1cs
dc.coverage.volume134cs
dc.date.issued2020-08-31cs
dc.description.abstractWe focused on the application of mass spectrometry and electrochemical methods combined with a chemometric analysis for the characterization of partially Zn(II)-loaded metallothionein-3 species. The results showed decreased Cat1 and Cat2 signals for the Zn(II)-loaded MT3 species with respect to the metal-free protein, which might be explained by the arrangement of tetrahedral metal-thiolate coordination environments and the formation of metal clusters. Moreover, there was a decrease in the Cat1 and Cat2 signals, and a plateau was reached with 4–5 Zn(II) ions that corresponded to the formation of the C-terminal a-domain. Regarding the Zn7-xMT3 complexes, we observed three different electrochemical behaviours for the Zn1–2MT3, Zn3–6MT3 and Zn7MT3 species. The difference for Zn1–2MT3 might be explained by the formation of independent ZnS4 cores in this stage that differ with respect to the formation of ZnxCysy clusters with an increased Zn(II) loading. The binding of the third Zn(II) ion to MT3 resulted in high sample heterogeneity due the co-existence of Zn3–6MT3. Finally, the Zn7MT3 protein showed a third type of behaviour. The fact that there were no free Cys residues might explain this phenomenon. Thus, this research identifies the major proteins responsible for zinc buffering in the cell.en
dc.formattextcs
dc.format.extent1-8cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationBIOELECTROCHEMISTRY. 2020, vol. 134, issue 1, p. 1-8.en
dc.identifier.doi10.1016/j.bioelechem.2020.107501cs
dc.identifier.issn0302-4598cs
dc.identifier.orcid0000-0002-8288-3999cs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.other164640cs
dc.identifier.researcheridN-9991-2014cs
dc.identifier.researcheridE11072012cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.scopus12040049600cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/195647
dc.language.isoencs
dc.publisherElseviercs
dc.relation.ispartofBIOELECTROCHEMISTRYcs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S1567539420300220cs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0302-4598/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectMetallomicsen
dc.subjectVoltammetryen
dc.subjectMetal ionsen
dc.subjectChemometricsen
dc.titleA chemometric-assisted voltammetric analysis of free and Zn(II)-loaded metallothionein-3 statesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionacceptedVersionen
sync.item.dbidVAV-164640en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:01en
sync.item.modts2025.01.17 15:14:49en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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