Platinum nanoparticles induce damage to DNA and inhibit DNA replication

Simple item page
dc.contributor.authorNejdl, Lukášcs
dc.contributor.authorKudr, Jiřícs
dc.contributor.authorMoulick, Amitavacs
dc.contributor.authorHegerová, Dagmarcs
dc.contributor.authorRuttkay-Nedecký, Branislavcs
dc.contributor.authorGumulec, Jaromírcs
dc.contributor.authorČíhalová, Kristýnacs
dc.contributor.authorŠmerková, Kristýnacs
dc.contributor.authorDostálová, Simonacs
dc.contributor.authorKřížková, Soňacs
dc.contributor.authorNovotná, Mariecs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorAdam, Vojtěchcs
dc.coverage.issue7cs
dc.coverage.volume12cs
dc.date.issued2017-07-12cs
dc.description.abstractSparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage , on the bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In vitro synthesized PtNPs were characterized by dynamic light scattering (PtNPs size range 4.8–11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry and atomic absorption spectrometry. The PtNPs inhibited the DNA replication and affected the secondary structure of DNA at higher concentrations, which was confirmed by polymerase chain reaction, DNA sequencing and DNA denaturation experiments. Further, cisplatin (CisPt), as traditional chemotherapy agent, was used in all parallel experiments. Moreover, the encapsulation of PtNPs in liposomes (LipoPtNPs) caused an approximately 2.4x higher of DNA damage in comparison with CisPt, LipoCisPt and PtNPs. The encapsulation of PtNPs in liposomes also increased their antibacterial, cytostatic and cytotoxic effect, which was determined by the method of growth curves on S. aureus and HFF cells. In addition, both the bare and encapsulated PtNPs caused lower oxidative stress (determined by GSH/GSSG ratio) in the human erythrocytes compared to the bare and encapsulated CisPt. CisPt was used in all parallel experiments as traditional chemotherapy agent.en
dc.formattextcs
dc.format.extent1-19cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPLOS ONE. 2017, vol. 12, issue 7, p. 1-19.en
dc.identifier.doi10.1371/journal.pone.0180798cs
dc.identifier.issn1932-6203cs
dc.identifier.orcid0000-0001-6332-8917cs
dc.identifier.orcid0000-0001-5769-6748cs
dc.identifier.orcid0000-0003-0826-234Xcs
dc.identifier.orcid0000-0002-9658-3444cs
dc.identifier.orcid0000-0002-1667-7660cs
dc.identifier.orcid0000-0002-0479-8369cs
dc.identifier.orcid0000-0003-4216-9544cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.other138521cs
dc.identifier.researcheridI-9677-2016cs
dc.identifier.researcheridJ-7030-2012cs
dc.identifier.researcheridD-7638-2012cs
dc.identifier.researcheridE-9617-2012cs
dc.identifier.researcheridE-5711-2012cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.scopus55783172600cs
dc.identifier.scopus55769747816cs
dc.identifier.scopus6603604023cs
dc.identifier.urihttp://hdl.handle.net/11012/70092
dc.language.isoencs
dc.publisherPLOScs
dc.relation.ispartofPLOS ONEcs
dc.relation.urihttp://europepmc.org/articles/PMC5507526?pdf=rendercs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1932-6203/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectPlatinum nanoparticlesen
dc.subjectDNA replicationen
dc.subjectDNA damageen
dc.titlePlatinum nanoparticles induce damage to DNA and inhibit DNA replicationen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-138521en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:24en
sync.item.modts2025.01.17 15:36:23en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
journal.pone.0180798.pdf
Size:
4.22 MB
Format:
Adobe Portable Document Format
Description:
journal.pone.0180798.pdf
Download
Collections
Chytré nanonástroje