Polarized Sonic Hedgehog Protein Localization and a Shift in the Expression of Region-Specific Molecules Is Associated With the Secondary Palate Development in the Veiled Chameleon

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dc.contributor.authorHampl, Marekcs
dc.contributor.authorDumková, Janacs
dc.contributor.authorKavková, Michaelacs
dc.contributor.authorDosedělová, Hanacs
dc.contributor.authorBryjová, Annacs
dc.contributor.authorZahradníček, Oldřichcs
dc.contributor.authorPyszko, Martincs
dc.contributor.authorMacholán, Milošcs
dc.contributor.authorZikmund, Tomášcs
dc.contributor.authorKaiser, Jozefcs
dc.contributor.authorBuchtová, Marcelacs
dc.coverage.issue1cs
dc.coverage.volume8cs
dc.date.accessioned2021-05-03T14:54:24Z
dc.date.available2021-05-03T14:54:24Z
dc.date.issued2020-07-28cs
dc.description.abstractSecondary palate development is characterized by the formation of two palatal shelves on the maxillary prominences, which fuse in the midline in mammalian embryos. However, in reptilian species, such as turtles, crocodilians, and lizards, the palatal shelves of the secondary palate develop to a variable extent and morphology. While in most Squamates, the palate is widely open, crocodilians develop a fully closed secondary palate. Here, we analyzed developmental processes that underlie secondary palate formation in chameleons, where large palatal shelves extend horizontally toward the midline. The growth of the palatal shelves continued during post-hatching stages and closure of the secondary palate can be observed in several adult animals. The massive proliferation of a multilayered oral epithelium and mesenchymal cells in the dorsal part of the palatal shelves underlined the initiation of their horizontal outgrowth, and was decreased later in development. The polarized cellular localization of primary cilia and Sonic hedgehog protein was associated with horizontal growth of the palatal shelves. Moreover, the development of large palatal shelves, supported by the pterygoid and palatine bones, was coupled with the shift inMeox2,Msx1, andPax9gene expression along the rostro-caudal axis. In conclusion, our results revealed distinctive developmental processes that contribute to the expansion and closure of the secondary palate in chameleons and highlighted divergences in palate formation across amniote species.en
dc.formattextcs
dc.format.extent572-1-572-29cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationFrontiers in Cell and Developmental Biology. 2020, vol. 8, issue 1, p. 572-1-572-29.en
dc.identifier.doi10.3389/fcell.2020.00572cs
dc.identifier.issn2296-634Xcs
dc.identifier.other169681cs
dc.identifier.urihttp://hdl.handle.net/11012/196660
dc.language.isoencs
dc.publisherFRONTIERS MEDIA SAcs
dc.relation.ispartofFrontiers in Cell and Developmental Biologycs
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fcell.2020.00572/fullcs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2296-634X/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectsecondary palateen
dc.subjectSHHen
dc.subjectprimary ciliaen
dc.subjectskeletogenesisen
dc.subjectchameleonen
dc.subjectreptileen
dc.titlePolarized Sonic Hedgehog Protein Localization and a Shift in the Expression of Region-Specific Molecules Is Associated With the Secondary Palate Development in the Veiled Chameleonen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-169681en
sync.item.dbtypeVAVen
sync.item.insts2021.05.03 16:54:24en
sync.item.modts2021.05.03 16:14:50en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Charakterizace materiálů a pokročilé povlaky 1-06cs
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