Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance

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dc.contributor.authorHúska, Daliborcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorMasařík, Michalcs
dc.contributor.authorHubálek, Jaromírcs
dc.contributor.authorTrnková, Libušecs
dc.contributor.authorKizek, Renécs
dc.coverage.issue3cs
dc.coverage.volume2009 (9)cs
dc.date.accessioned2017-10-12T10:46:08Z
dc.date.available2017-10-12T10:46:08Z
dc.date.issued2009-03-15cs
dc.description.abstractTreatment strategies for tumour diseases are progressively focusing on personalization of medicine. However, this focus requires methods revealing the early general biological mechanisms, including the formation anti-cancer drugs' resistance. The low molecular mass protein metallothionein is thought to be the crucial for the formation of resistance in tumour treatment based on the platinum-cytostatics. The interactions between metallothionein (MT) and cisplatin were determined by the adsorptive transfer stripping technique coupled with the differential pulse votlammetry Brdicka's reaction. The signals related to the MT-cisplatin complex appeared at -0.9 V. The formation of this complex depended on the time of interaction between cisplatin and MT. The complex formation was consequently confirmed by quartz crystal microbalance analyses. The formation of this complex was detectable even after a 20 s long interaction. Moreover, we detected presence of MT-cisplatin complex in the blood of male rats treated with this drug.en
dc.formattextcs
dc.format.extent1355-1369cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationSENSORS. 2009, vol. 2009 (9), issue 3, p. 1355-1369.en
dc.identifier.doi10.3390/s90301355cs
dc.identifier.issn1424-8220cs
dc.identifier.other48743cs
dc.identifier.urihttp://hdl.handle.net/11012/70083
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofSENSORScs
dc.relation.urihttp://www.mdpi.com/1424-8220/9/3/1355cs
dc.rightsCreative Commons Attribution 3.0 Unportedcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1424-8220/cs
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/cs
dc.subjectCanceren
dc.subjectMetallothioneinen
dc.subjectCisplatinen
dc.subjectProtein-Drug Interactionen
dc.subjectVoltammetryen
dc.subjectBrdicka's reactionen
dc.subjectQuartz Crystal Microbalanceen
dc.titleStudy of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalanceen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-48743en
sync.item.dbtypeVAVen
sync.item.insts2020.03.31 09:56:54en
sync.item.modts2020.03.31 07:43:58en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav mikroelektronikycs
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