Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium

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dc.contributor.authorPernicová, Ivacs
dc.contributor.authorNováčková, Ivanacs
dc.contributor.authorSedláček, Petrcs
dc.contributor.authorKouřilová, Xeniecs
dc.contributor.authorKalina, Michalcs
dc.contributor.authorKovalčík, Adriánacs
dc.contributor.authorKoller, Martincs
dc.contributor.authorNebesářová, Janacs
dc.contributor.authorKrzyžánek, Vladislavcs
dc.contributor.authorHrubanová, Kamilacs
dc.contributor.authorMásilko, Jiřícs
dc.contributor.authorSlaninová, Evacs
dc.contributor.authorObruča, Stanislavcs
dc.coverage.issue6cs
dc.coverage.volume12cs
dc.date.accessioned2020-08-04T10:59:44Z
dc.date.available2020-08-04T10:59:44Z
dc.date.issued2020-05-29cs
dc.description.abstractExtremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolateAneurinibacillussp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 degrees C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated thatAneurinibacillussp. H1 is a very promising candidate for production of PHA with tailored material properties.en
dc.formattextcs
dc.format.extent1-13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPolymers. 2020, vol. 12, issue 6, p. 1-13.en
dc.identifier.doi10.3390/polym12061235cs
dc.identifier.issn2073-4360cs
dc.identifier.other164652cs
dc.identifier.urihttp://hdl.handle.net/11012/193675
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofPolymerscs
dc.relation.urihttps://www.mdpi.com/2073-4360/12/6/1235cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2073-4360/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectpolyhydroxyalkanoatesen
dc.subjectthermophilesen
dc.subjectAneurinibacillusspen
dc.subjectP(3HB-co-4HB)en
dc.subjectP(3HB-co-3HV-co-4HB)en
dc.titleIntroducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacteriumen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-164652en
sync.item.dbtypeVAVen
sync.item.insts2020.11.19 12:53:04en
sync.item.modts2020.11.19 12:14:17en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Centrum materiálového výzkumucs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav fyzikální a spotřební chemiecs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie materiálůcs
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