The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins–Metal Complexes

dc.contributor.authorMerlos Rodrigo, Miguel Ángelcs
dc.contributor.authorJimenez Jimenez, Ana Mariacs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorRichtera, Lukášcs
dc.contributor.authorAdam, Vojtěchcs
dc.coverage.issue3cs
dc.coverage.volume18cs
dc.date.issued2017-03-01cs
dc.description.abstractThe translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, with multiple isoforms within each class. The main aim of this study was to determine the behavior of MT in dependence on various externally modelled environments, using electrochemistry. In our study, the mass distribution of MTs was characterized using MALDI-TOF. After that, adsorptive transfer stripping technique with differential pulse voltammetry was selected for optimization of electrochemical detection of MTs with regard to accumulation time and pH effects. Our results show that utilization of 0.5 M NaCl, pH 6.4, as the supporting electrolyte provides a highly complicated fingerprint, showing a number of non-resolved voltammograms. Hence, we further resolved the voltammograms exhibiting the broad and overlapping signals using curve fitting. The separated signals were assigned to the electrochemical responses of several MT complexes with zinc(II), cadmium(II), and copper(II), respectively. Our results show that electrochemistry could serve as a great tool for metalloproteomic applications to determine the ratio of metal ion bonds within the target protein structure, however, it provides highly complicated signals, which require further resolution using a proper statistical method, such as curve fitting.en
dc.formattextcs
dc.format.extent1-11cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Molecular Sciences. 2017, vol. 18, issue 3, p. 1-11.en
dc.identifier.doi10.3390/ijms18030610cs
dc.identifier.issn1422-0067cs
dc.identifier.orcid0000-0003-1736-492Xcs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.orcid0000-0002-8288-3999cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.other135984cs
dc.identifier.researcheridE11072012cs
dc.identifier.researcheridN-9991-2014cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.scopus14012648400cs
dc.identifier.scopus12040049600cs
dc.identifier.urihttp://hdl.handle.net/11012/69371
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofInternational Journal of Molecular Sciencescs
dc.relation.urihttp://www.mdpi.com/1422-0067/18/3/610cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1422-0067/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectelectrochemistryen
dc.subjectmass spectrometry MALDI-TOFen
dc.subjectmetallothioneinen
dc.subjectmetallomicsen
dc.subjectsignal resolvingen
dc.titleThe Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins–Metal Complexesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-135984en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:39:16en
sync.item.modts2025.01.17 15:14:53en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. oddělení-MEL-SIXcs
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