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Antibacterial activity of composite of graphene oxide with silver nanoparticles
(Mendel University in Brno, 2017-12-31) Bytešníková, Zuzana; Koudelková, Zuzana; Richtera, Lukáš; Kopel, Pavel; Adam, Vojtěch
Looking for strategies against the development of antibiotic resistance is a major global object of interest for the public health. This work deals with synthesis of antimicrobial composites of graphene oxide (GO) with metal nanoparticles. GO has been prepared by modified Hummers' method and characterized using scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR) and differential pulse voltammetry. Composites of GO have been synthetized with silve nanoparticles, which have been characterized. Potential antimicrobial activity of the nanocomposites was tested against Escherichia coli, Staphylococcus aureus and methicilin resistant Staphylococcus aureus (MRSA).
DEP-on-a-Chip: Dielectrophoresis Applied to Microfluidic Platforms
(MDPI, 2019-06-24) Zhang, Haoqing; Chang, Honglong; Neužil, Pavel
Dielectric particles in a non-uniform electric field are subject to a force caused by a phenomenon called dielectrophoresis (DEP). DEP is a commonly used technique in microfluidics for particle or cell separation. In comparison with other separation methods, DEP has the unique advantage of being label-free, fast, and accurate. It has been widely applied in microfluidics for bio-molecular diagnostics and medical and polymer research. This review introduces the basic theory of DEP, its advantages compared with other separation methods, and its applications in recent years, in particular, focusing on the different electrode types integrated into microfluidic chips, fabrication techniques, and operation principles.
High-Conductivity Stoichiometric Titanium Nitride for Bioelectronics
(Wiley-VCH GmbH, 2023-02-02) Gablech, Imrich; Migliaccio, Ludovico; Brodský, Jan; Havlíček, Marek; Podešva, Pavel; Hrdý, Radim; Ehlich, Jiří; Gryszel, Maciej; Glowacki, Eric Daniel
Bioelectronic devices such as neural stimulation and recording devices require stable low-impedance electrode interfaces. Various forms of nitridated titanium are used in biointerface applications due to robustness and biological inertness. In this work, stoichiometric TiN thin films are fabricated using a dual Kaufman ion-beam source setup, without the necessity of substrate heating. These layers are remarkable compared to established forms of TiN due to high degree of crystallinity and excellent electrical conductivity. How this fabrication method can be extended to produce structured AlN, to yield robust AlN/TiN bilayer micropyramids, is described. These electrodes compare favorably to commercial TiN microelectrodes in the performance metrics important for bioelectronics interfaces: higher conductivity (by an order of magnitude), lower electrochemical impedance, and higher capacitive charge injection with lower faradaicity. These results demonstrate that the Kaufman ion-beam sputtering method can produce competitive nitride ceramics for bioelectronics applications at low deposition temperatures.
Love wave sensors based on gold nanoparticle-modified polypyrrole and their properties to ammonia and ethylene
(Elsevier, 2020-02-01) Šetka, Milena; Potoček, Michal; Král, Zdeněk; Drbohlavová, Jana; Vallejos Vargas, Stella
This work presents the fabrication and gas sensing properties of Love wave sensors based on non-modified and gold nanoparticle-modified polypyrrole. Results demonstrate the integration of uniform polypyrrole layers with smooth granular surface and the incorporation of dispersed crystalline gold nanoparticles within the modified layers. Gas sensing tests of the sensors in dry conditions show enhanced sensing performance to ethylene and ammonia for the modified systems as compared to those without modification and those reported in the literature. The effect of humidity proves significant in both systems with the results showing a decrease of sensitivity in humid conditions. Despite this weakness, the relatively facile and scalable fabrication of these sensors, as well as their sensing response at room temperature may be attractive in gas detection systems, in which high humidity levels can be restricted by the use of filter or preconditioning elements.
Influence of liposomal platinum cytostatics on cancer cells by voltammetric methods
(TANGER, 2018-03-31) Dostálová, Eliška; Laníková, Petra; Hynek, David; Krejčová, Ludmila; Michálková, Hana; Richtera, Lukáš; Adam, Vojtěch
Aim of this work deals with closing of cisplatin into liposomes, the study of their stability and interaction with cancer cell lines. Commercial cisplatin cytostatic was encapsulated into three types of liposomes (L8, L10 and L15) of a different chemical composition. Each of liposomes had different ability to close cytostatic. L8 differs from L10 by containing cholesterol. In despite of L10 and L8, L15 liposome contains a synthetic 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (C41H78NO8P) – DOPE, which is generally found in cell membranes. The size of the liposomes was characterized by Dynamic Light Scattering (DLS). The amount of Pt-drug in liposomes was verified by electrochemical determination. Their stability was observed by differential pulse voltammetry (DPV). These studies also demonstrated, that the cisplatin is stable in physiological solution for at least 24 hours, which making it a suitable medium storage for this drug. Finally, the encapsulated cisplatin was applied to cancer cell lines and their viability was observed by MTT assay.

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