Sarcosine degradation pathway is involved in the epigenetics of prostate cells

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dc.contributor.authorStrmiska, Vladislavcs
dc.contributor.authorMichálek, Petrcs
dc.contributor.authorBuchtelová, Hanacs
dc.contributor.authorLacková, Zuzanacs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorKřížková, Soňacs
dc.contributor.authorPompeiano Vaníčková, Luciecs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorStiborová, Mariecs
dc.contributor.authorEckschlager, Tomášcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorHeger, Zbyněkcs
dc.date.accessioned2018-08-23T10:52:54Z
dc.date.available2018-08-23T10:52:54Z
dc.date.issued2017-12-31cs
dc.description.abstractIt has been shown that sarcosine suplementation stimulates the proliferation of prostate cells and also their invassiveness. In present study we show that enzymes conected with sarcosine conversion to glycine (sarcosine dehydrogenase, pipecolic acid oxidase) are stimulated due to sarcosine treatment. Further, sarcosine treatment increases S-adenosylmethioneine-to-S-adenosylhomocysteine ratio, which indicates a release and utilization of free methyl groups from sarcosine degradation pathway. We identified the highest induction of global methylation in non-malignant PNT1A cells, but global methylation profiles were altered also in malignant (22Rv1) and metastatic (LNCaP) cells. The influence on methylation changes was further verified using hypomethylating agent 5-azacytidine (5-aza). Co-treatment of prostate cells with 5-aza and sarcosine resulted in decrease in cells invassiveness when compared to treatment with sarcosine alone. This correlates with sarcosine-related hypermethylation of genes involved in cells growth and cell cycle.en
dc.formattextcs
dc.format.extent927-931cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2017. 2017, p. 927-931.en
dc.identifier.isbn978-80-7509-529-9cs
dc.identifier.other148758cs
dc.identifier.urihttp://hdl.handle.net/11012/84186
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2017cs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201701-0108_Sarcosine-degradation-pathway-is-involved-in-the-epigenetics-of-prostate-cells.php?back=/magno/mnt/2017/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectSarcosineen
dc.subjectmethylationen
dc.subjectprostateen
dc.subjectprostate canceren
dc.subjecthuman cellsen
dc.titleSarcosine degradation pathway is involved in the epigenetics of prostate cellsen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-148758en
sync.item.dbtypeVAVen
sync.item.insts2020.04.01 11:01:49en
sync.item.modts2020.04.01 05:59:38en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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