Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

Simple item page
dc.contributor.authorKroupová, Zuzanacs
dc.contributor.authorSlaninová, Evacs
dc.contributor.authorMrázová, Kateřinacs
dc.contributor.authorKrzyžánek, Vladislavcs
dc.contributor.authorHrubanová, Kamilacs
dc.contributor.authorFritz, Inescs
dc.contributor.authorObruča, Stanislavcs
dc.coverage.issue11cs
dc.coverage.volume70cs
dc.date.accessioned2025-04-08T09:56:16Z
dc.date.available2025-04-08T09:56:16Z
dc.date.issued2024-11-06cs
dc.description.abstractCyanobacteria are prokaryotic organisms characterised by their complex structures and a wide range of pigments. With their ability to fix CO2, cyanobacteria are interesting for white biotechnology as cell factories to produce various high-value metabolites such as polyhydroxyalkanoates, pigments, or proteins. White biotechnology is the industrial production and processing of chemicals, materials, and energy using microorganisms. It is known that exposing cyanobacteria to low levels of stressors can induce the production of secondary metabolites. Understanding of this phenomenon, known as hormesis, can involve the strategic application of controlled stressors to enhance the production of specific metabolites. Consequently, precise measurement of cyanobacterial viability becomes crucial for process control. However, there is no established reliable and quick viability assay protocol for cyanobacteria since the task is challenging due to strong interferences of autofluorescence signals of intercellular pigments and fluorescent viability probes when flow cytometry is used. We performed the screening of selected fluorescent viability probes used frequently in bacteria viability assays. The results of our investigation demonstrated the efficacy and reliability of three widely utilised types of viability probes for the assessment of the viability of Synechocystis strains. The developed technique can be possibly utilised for the evaluation of the importance of polyhydroxyalkanoates for cyanobacterial cultures with respect to selected stressor-repeated freezing and thawing. The results indicated that the presence of polyhydroxyalkanoate granules in cyanobacterial cells could hypothetically contribute to the survival of repeated freezing and thawing.en
dc.formattextcs
dc.format.extent205-223cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationFOLIA MICROBIOLOGICA. 2024, vol. 70, issue 11, p. 205-223.en
dc.identifier.doi10.1007/s12223-024-01212-wcs
dc.identifier.issn1874-9356cs
dc.identifier.orcid0000-0002-8956-6515cs
dc.identifier.orcid0000-0002-1808-8632cs
dc.identifier.orcid0000-0002-9270-195Xcs
dc.identifier.other193545cs
dc.identifier.scopus25621797900cs
dc.identifier.urihttps://hdl.handle.net/11012/250841
dc.language.isoencs
dc.publisherSringercs
dc.relation.ispartofFOLIA MICROBIOLOGICAcs
dc.relation.urihttps://link.springer.com/article/10.1007/s12223-024-01212-wcs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1874-9356/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectBiotechnologyen
dc.subjectCyanobacteriaen
dc.subjectFlow cytometryen
dc.subjectFluorescent viability probesen
dc.subjectStress resistanceen
dc.subjectViability assessmenten
dc.titleEvaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessmenten
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-193545en
sync.item.dbtypeVAVen
sync.item.insts2025.04.08 11:56:16en
sync.item.modts2025.04.08 11:33:23en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Centrum materiálového výzkumucs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s1222302401212w.pdf
Size:
2.11 MB
Format:
Adobe Portable Document Format
Description:
file s1222302401212w.pdf
Download
Collections
Ústav chemie potravin a biotechnologií
Centrum materiálového výzkumu