Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis

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dc.contributor.authorKouřilová, Xeniecs
dc.contributor.authorBuchtíková, Ivacs
dc.contributor.authorVidláková, Michaelacs
dc.contributor.authorKrejčiřík, Romancs
dc.contributor.authorMrázová, Kateřinacs
dc.contributor.authorHrubanová, Kamilacs
dc.contributor.authorKrzyžánek, Vladislavcs
dc.contributor.authorNebesářová, Janacs
dc.contributor.authorObruča, Stanislavcs
dc.coverage.issue10cs
dc.coverage.volume8cs
dc.date.issued2021-09-01cs
dc.description.abstractPolyhydroxyalkanoates (PHA) are microbial polyesters that have recently come to the forefront of interest due to their biodegradability and production from renewable sources. A potential increase in competitiveness of PHA production process comes with a combination of the use of thermophilic bacteria with the mutual use of waste substrates. In this work, the thermophilic bacterium Tepidimonas taiwanensis LMG 22826 was identified as a promising PHA producer. The ability to produce PHA in T. taiwanensis was studied both on genotype and phenotype levels. The gene encoding the Class I PHA synthase, a crucial enzyme in PHA synthesis, was detected both by genome database search and by PCR. The microbial culture of T. taiwanensis was capable of efficient utilization of glucose and fructose. When cultivated on glucose as the only carbon source at 50 & DEG;C, the PHA titers reached up to 3.55 g/L, and PHA content in cell dry mass was 65%. The preference of fructose and glucose opens the possibility to employ T. taiwanensis for PHA production on various food wastes rich in these abundant sugars. In this work, PHA production on grape pomace extracts was successfully tested.</p>en
dc.formattextcs
dc.format.extent1-13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationBioengineering. 2021, vol. 8, issue 10, p. 1-13.en
dc.identifier.doi10.3390/bioengineering8100141cs
dc.identifier.issn2306-5354cs
dc.identifier.orcid0000-0002-1339-6660cs
dc.identifier.orcid0000-0002-9528-5445cs
dc.identifier.orcid0000-0002-9270-195Xcs
dc.identifier.other175573cs
dc.identifier.scopus57200035491cs
dc.identifier.scopus25621797900cs
dc.identifier.urihttp://hdl.handle.net/11012/203349
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofBioengineeringcs
dc.relation.urihttps://www.mdpi.com/2306-5354/8/10/141cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2306-5354/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectpolyhydroxyalkanoatesen
dc.subjectTepidimonas taiwanensisen
dc.subjectgrape pomaceen
dc.subjectthermophilesen
dc.titleBiotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensisen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-175573en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:37:58en
sync.item.modts2025.01.17 16:33:35en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Fakulta chemickács
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Centrum materiálového výzkumucs
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Ústav chemie potravin a biotechnologií