SPEED: an integrated, smartphone-operated, handheld digital PCR Device for point-of-care testing

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dc.contributor.authorZhang, Haoqingcs
dc.contributor.authorLiu, Xiaochengcs
dc.contributor.authorWang, Xinlucs
dc.contributor.authorYan, Zhiqiangcs
dc.contributor.authorXu, Yingcs
dc.contributor.authorGaňová, Martinacs
dc.contributor.authorŘezníček, Tomášcs
dc.contributor.authorKorabečná, Mariecs
dc.contributor.authorNeužil, Pavelcs
dc.coverage.issue1cs
dc.coverage.volume10cs
dc.date.issued2024-05-20cs
dc.description.abstractThis study elaborates on the design, fabrication, and data analysis details of SPEED, a recently proposed smartphone-based digital polymerase chain reaction (dPCR) device. The dPCR chips incorporate partition diameters ranging from 50 mu m to 5 mu m, and these partitions are organized into six distinct blocks to facilitate image processing. Due to the superior thermal conductivity of Si and its potential for mass production, the dPCR chips were fabricated on a Si substrate. A temperature control system based on a high-power density Peltier element and a preheating/cooling PCR protocol user interface shortening the thermal cycle time. The optical design employs four 470 nm light-emitting diodes as light sources, with filters and mirrors effectively managing the light emitted during PCR. An algorithm is utilized for image processing and illumination nonuniformity correction including conversion to a monochromatic format, partition identification, skew correction, and the generation of an image correction mask. We validated the device using a range of deoxyribonucleic acid targets, demonstrating its potential applicability across multiple fields. Therefore, we provide guidance and verification of the design and testing of the recently proposed SPEED device.en
dc.formattextcs
dc.format.extent10cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMicrosystems & Nanoengineering. 2024, vol. 10, issue 1, 10 p.en
dc.identifier.doi10.1038/s41378-024-00689-2cs
dc.identifier.issn2055-7434cs
dc.identifier.other188771cs
dc.identifier.urihttp://hdl.handle.net/11012/249360
dc.language.isoencs
dc.publisherSpringer Naturecs
dc.relation.ispartofMicrosystems & Nanoengineeringcs
dc.relation.urihttps://www.nature.com/articles/s41378-024-00689-2cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2055-7434/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectQUANTIFICATIONen
dc.subjectDIAGNOSISen
dc.titleSPEED: an integrated, smartphone-operated, handheld digital PCR Device for point-of-care testingen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-188771en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:41:20en
sync.item.modts2025.01.17 15:28:12en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav mikroelektronikycs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Laserová spektroskopiecs
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