A Comparison of the Effects of Continuous Illumination and Day/Night Regimes on PHB Accumulation in Synechocystis Cells

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dc.contributor.authorFleischhacker-Daffert, Christinacs
dc.contributor.authorZerobin, Antoniacs
dc.contributor.authorHummel, Ferdinandcs
dc.contributor.authorSlaninová, Evacs
dc.contributor.authorKroupová, Zuzanacs
dc.contributor.authorObruča, Stanislavcs
dc.contributor.authorMrázová, Kateřinacs
dc.contributor.authorHrubanová, Kamilacs
dc.contributor.authorKrzyžánek, Vladislavcs
dc.contributor.authorNebesářová, Janacs
dc.contributor.authorLudwig, Katharinacs
dc.contributor.authorFritz, Inescs
dc.coverage.issue7cs
dc.coverage.volume14cs
dc.date.accessioned2025-02-28T13:53:48Z
dc.date.available2025-02-28T13:53:48Z
dc.date.issued2024-07-20cs
dc.description.abstractPoly(3-hydroxybutyrate) (PHB) is a biobased and biodegradable polymer with properties comparable to polypropylene and therefore has the potential to replace conventional plastics. PHB is intracellularly accumulated by prokaryotic organisms. For the cells PHB functions manly as carbon and energy source, but all possible functions of PHB are still not known. Synechocystis (cyanobacteria) accumulates PHB using light as energy and CO2 as carbon source. The main trigger for PHB accumulation in cyanobacteria is nitrogen and phosphorous depletion with simultaneous surplus of carbon and energy. For the above reasons, obtaining knowledge about external factors influencing PHB accumulation is of highest interest. This study compares the effect of continuous light exposure and day/night (16/8 h) cycles on selected physiology parameters of three Synechocystis strains. We show that continuous illumination at moderate light intensities leads to an increased PHB accumulation in Synechocystis salina CCALA 192 (max. 14.2% CDW - cell dry weight) compared to day/night cycles (3.7% CDW). In addition to PHB content, glycogen and cell size increased, while cell density and cell viability decreased. The results offer new approaches for further studies to gain deeper insights into the role of PHB in cyanobacteria to obtain bioplastics in a more sustainable and environmentally friendly way.en
dc.formattextcs
dc.format.extent1-18cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationLife. 2024, vol. 14, issue 7, p. 1-18.en
dc.identifier.doi10.3390/life14070907cs
dc.identifier.issn2075-1729cs
dc.identifier.orcid0000-0002-1808-8632cs
dc.identifier.orcid0000-0002-8956-6515cs
dc.identifier.orcid0000-0002-9270-195Xcs
dc.identifier.other191234cs
dc.identifier.scopus25621797900cs
dc.identifier.urihttps://hdl.handle.net/11012/250074
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofLifecs
dc.relation.urihttps://www.mdpi.com/2075-1729/14/7/907cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2075-1729/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectSynechocystisen
dc.subjectcontinuous illuminationen
dc.subjectday/night cycleen
dc.subjectPHBen
dc.subjectglycogenen
dc.subjectcell sizeen
dc.titleA Comparison of the Effects of Continuous Illumination and Day/Night Regimes on PHB Accumulation in Synechocystis Cellsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-191234en
sync.item.dbtypeVAVen
sync.item.insts2025.02.28 14:53:48en
sync.item.modts2025.02.28 13:32:00en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Centrum materiálového výzkumucs
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