Organic films with amino groups on nanofibrous structure control innate immune response

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dc.contributor.authorJanůšová, Martinacs
dc.contributor.authorMatušů, Patrikcs
dc.contributor.authorBartošíková, Janacs
dc.contributor.authorJanů, Luciecs
dc.contributor.authorŠillerová, Zdeňkacs
dc.contributor.authorNečas, Davidcs
dc.contributor.authorRyšánek, Petrcs
dc.contributor.authorMedalová, Jiřinacs
dc.contributor.authorZajíčková, Lenkacs
dc.coverage.issueDecembercs
dc.coverage.volume47cs
dc.date.accessioned2025-10-31T07:05:17Z
dc.date.available2025-10-31T07:05:17Z
dc.date.issued2025-12-01cs
dc.description.abstractSynthetic polymer nanofibers, such as polycaprolactone (PCL), are widely used as scaffolds in tissue engineering due to their ease of fabrication. However, their surface properties often fail to meet the specific requirements of cell culture. Coating the scaffolds with organic thin films containing amino groups using a plasma-based dry method provides a simple, single-step, and tunable approach to enhance their hydrophilicity. Motivated by the potential application of these films in bioengineering, this study investigates key characteristics influencing the immune response, capturing both the early and late stages of immune activation represented here by neutrophil-like and macrophage-like cell models, respectively. In neutrophils, the strongest undesirable pro- inflammatory activation was triggered by films with high nitrogen content, positive surface charge, and low water stability, as evidenced by elevated expression of pro-inflammatory cytokines. Macrophage-like cells exhibited a similar trend, albeit with a shifted activation threshold: amine films characterized by lower nitrogen content more effectively reduced pro-inflammatory activation. Morphological changes in macrophage-like cells further supported the role of surface chemistry in modulating their behavior. In addition to surface chemistry, substrate morphology played a role in immune modulation. The porous structure of PCL nanofibers enhanced the immune profile of macrophage-like cells by increasing pro-regenerative M2 cytokine expression and reducing pro-inflammatory M1 markers. In contrast, neutrophil-like cells were largely unaffected by substrate morphology and responded primarily to surface chemistry. This study underscores the importance of immune response investigation in biomaterial design.en
dc.description.abstractSynthetic polymer nanofibers, such as polycaprolactone (PCL), are widely used as scaffolds in tissue engineering due to their ease of fabrication. However, their surface properties often fail to meet the specific requirements of cell culture. Coating the scaffolds with organic thin films containing amino groups using a plasma-based dry method provides a simple, single-step, and tunable approach to enhance their hydrophilicity. Motivated by the potential application of these films in bioengineering, this study investigates key characteristics influencing the immune response, capturing both the early and late stages of immune activation represented here by neutrophil-like and macrophage-like cell models, respectively. In neutrophils, the strongest undesirable pro- inflammatory activation was triggered by films with high nitrogen content, positive surface charge, and low water stability, as evidenced by elevated expression of pro-inflammatory cytokines. Macrophage-like cells exhibited a similar trend, albeit with a shifted activation threshold: amine films characterized by lower nitrogen content more effectively reduced pro-inflammatory activation. Morphological changes in macrophage-like cells further supported the role of surface chemistry in modulating their behavior. In addition to surface chemistry, substrate morphology played a role in immune modulation. The porous structure of PCL nanofibers enhanced the immune profile of macrophage-like cells by increasing pro-regenerative M2 cytokine expression and reducing pro-inflammatory M1 markers. In contrast, neutrophil-like cells were largely unaffected by substrate morphology and responded primarily to surface chemistry. This study underscores the importance of immune response investigation in biomaterial design.en
dc.formattextcs
dc.format.extent1-16cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationApplied Materials Today. 2025, vol. 47, issue December, p. 1-16.en
dc.identifier.doi10.1016/j.apmt.2025.102965cs
dc.identifier.issn2352-9407cs
dc.identifier.orcid0000-0003-0399-0241cs
dc.identifier.orcid0009-0007-8171-6025cs
dc.identifier.orcid0000-0002-2732-3681cs
dc.identifier.orcid0000-0002-6298-2228cs
dc.identifier.orcid0009-0006-4063-5336cs
dc.identifier.orcid0000-0001-7731-8453cs
dc.identifier.orcid0000-0002-9209-3202cs
dc.identifier.orcid0000-0002-7377-2840cs
dc.identifier.orcid0000-0002-6906-8906cs
dc.identifier.other199300cs
dc.identifier.researcheridAAD-5562-2022cs
dc.identifier.researcheridD-7166-2012cs
dc.identifier.researcheridE-3010-2012cs
dc.identifier.scopus22933742100cs
dc.identifier.urihttps://hdl.handle.net/11012/255615
dc.language.isoencs
dc.relation.ispartofApplied Materials Todaycs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S235294072500383Xcs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2352-9407/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectAmino-functionalized surfacesen
dc.subjectPolymer nanofibersen
dc.subjectImmune responseen
dc.subjectNeutrophilsen
dc.subjectMacrophagesen
dc.subjectAmino-functionalized surfaces
dc.subjectPolymer nanofibers
dc.subjectImmune response
dc.subjectNeutrophils
dc.subjectMacrophages
dc.titleOrganic films with amino groups on nanofibrous structure control innate immune responseen
dc.title.alternativeOrganic films with amino groups on nanofibrous structure control innate immune responseen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
eprints.grantNumberinfo:eu-repo/grantAgreement/GA0/GA/GA24-12321Scs
sync.item.dbidVAV-199300en
sync.item.dbtypeVAVen
sync.item.insts2025.10.31 08:05:17en
sync.item.modts2025.10.31 07:33:10en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Plazmové technologie pro materiálycs
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