Novel Electrochemical Biosensor for Simultaneous Detection of Adenine and Guanine Based on Cu2O Nanoparticles
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Date
2012-11-02
Authors
Pekárková, Jana
Prášek, Jan
Majzlíková, Petra
Trnková, Libuše
Drbohlavová, Jana
Pekárek, Jan
Hrdý, Radim
Hubálek, Jaromír
0000-0001-9705-1565Advisor
Referee
Mark
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Elsevier
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Abstract
In this paper Cu2O nanoparticles were prepared and used for the construction of novel electrochemical voltammetric biosensor for the simultaneous detection of adenine and guanine. Cu2O nanoparticles were synthesized via simple wet chemical route, where glucose was used as a reductant. The nanoparticles were characterized by SEM and XRD analysis, which showed the presence of spherical aggregations with diameter of 1000 nm. These nanoparticles were successfully used for fabrication of spray-coated and screen-printed working electrodes on alumina substrate for the electrochemical detection of purine bases. We observed that Cu(I) reacts with adenine to form insoluble complex that accumulates on the electrode surface and causes the decrease of current response. In the case of guanine, we did not observed any significant decrease of current response which is probably caused by adsorption of guanine on the electrode surface.
In this paper Cu2O nanoparticles were prepared and used for the construction of novel electrochemical voltammetric biosensor for the simultaneous detection of adenine and guanine. Cu2O nanoparticles were synthesized via simple wet chemical route, where glucose was used as a reductant. The nanoparticles were characterized by SEM and XRD analysis, which showed the presence of spherical aggregations with diameter of 1000 nm. These nanoparticles were successfully used for fabrication of spray-coated and screen-printed working electrodes on alumina substrate for the electrochemical detection of purine bases. We observed that Cu(I) reacts with adenine to form insoluble complex that accumulates on the electrode surface and causes the decrease of current response. In the case of guanine, we did not observed any significant decrease of current response which is probably caused by adsorption of guanine on the electrode surface.
In this paper Cu2O nanoparticles were prepared and used for the construction of novel electrochemical voltammetric biosensor for the simultaneous detection of adenine and guanine. Cu2O nanoparticles were synthesized via simple wet chemical route, where glucose was used as a reductant. The nanoparticles were characterized by SEM and XRD analysis, which showed the presence of spherical aggregations with diameter of 1000 nm. These nanoparticles were successfully used for fabrication of spray-coated and screen-printed working electrodes on alumina substrate for the electrochemical detection of purine bases. We observed that Cu(I) reacts with adenine to form insoluble complex that accumulates on the electrode surface and causes the decrease of current response. In the case of guanine, we did not observed any significant decrease of current response which is probably caused by adsorption of guanine on the electrode surface.
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Citation
Procedia Engineering. 2012, vol. 47, issue 11, p. 702-705.
https://www.sciencedirect.com/science/article/pii/S187770581204307X
https://www.sciencedirect.com/science/article/pii/S187770581204307X
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Peer-reviewed
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en
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Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported
http://creativecommons.org/licenses/by-nc-nd/3.0/
http://creativecommons.org/licenses/by-nc-nd/3.0/