Effect of lycorine on the green algae Chlamydomonas reinhardtii under UV-C irradiation

Abstract

Lycorin (LYC) is an inhibitor of the growth in higher plants through the inhibition of the last step of ascorbic acid biosynthesis. Ascorbates are mainly involved in regulation of intracellular levels reactive oxygen species. There is still limit information about its green microalgal toxicity and specificity. Therefore, present experiment was focused on the LYC toxicity on the green microalgae, Chlamydomonas reinhardtii, moreover the cells of microalgae were exposed to UV-C irradiation to increase ROS. The attention was primary given to the antioxidant response. The higher concentrations than 25 M LYC with 30 min UV-C (250 nm) exposure absolutely inhibited the growth. 10 M LYC and 25 M LYC treatment together with UV-C irradiation suppress the growth caused oxidation stress and enhances antioxidant response.Lycorin (LYC) is an inhibitor of the growth in higher plants through the inhibition of the last step of ascorbic acid biosynthesis. Ascorbates are mainly involved in regulation of intracellular levels reactive oxygen species. There is still limit information about its green microalgal toxicity and specificity. Therefore, present experiment was focused on the LYC toxicity on the green microalgae, Chlamydomonas reinhardtii, moreover the cells of microalgae were exposed to UV-C irradiation to increase ROS. The attention was primary given to the antioxidant response. The higher concentrations than 25 M LYC with 30 min UV-C (250 nm) exposure absolutely inhibited the growth. 10 M LYC and 25 M LYC treatment together with UV-C irradiation suppress the growth caused oxidation stress and enhances antioxidant response.