Chick chorioallantoic membrane (CAM) assay for the evaluation of the antitumor and antimetastatic activity of platinum-based drugs in association with the impact on the amino acid metabolism

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dc.contributor.authorMitrevska, Katerinacs
dc.contributor.authorMerlos Rodrigo, Miguel Ángelcs
dc.contributor.authorCernei, Natalia Vladimirovnacs
dc.contributor.authorMichálková, Hanacs
dc.contributor.authorŠplíchal, Zbyněkcs
dc.contributor.authorHynek, Davidcs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorHeger, Zbyněkcs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorMilosavljević, Vedrancs
dc.coverage.issue4cs
dc.coverage.volume19cs
dc.date.issued2023-04-01cs
dc.description.abstractThe combination of in ovo and ex ovo chorioallantoic membrane (CAM) assay provides an excellent platform which extends its relevance in studying carcinogenesis to the field of screening of anticancer activity of platinum nanoparticles (PtNPs) and further study of the amino acids' fluctuations in liver and brain. PtNPs are promising candidates for replacing cisplatin (CDDP); however, insufficient data of their antitumor efficiency and activity on the cancer-related amino acid metabolism are available, and the assessment of the in vivo performance has barely scratched the surface. Herein, we used CAM assay as in vivo model for screening of novel therapeutic modalities, and we conducted a comparative study of the effects of CDDP and polyvinylpyrrolidone coated PtNPs on MDA-MB-231 breast cancer xenograft. PtNPs showed a higher efficiency to inhibit the tumor growth and metastasis compared to CDDP. The amino acids profiling in the MDA-MB-231 cells revealed that the PtNPs had an overall depleting effect on the amino acids content. Noteworthy, more side effects to amino acid metabolism were deduced from the depletion of the amino acids in tumor, brain, and liver upon CDDP treatment. Different sets of enzymes of the tricarboxylic acid (TCA) cycle were targeted by PtNPs and CDDP, and while mRNA encoding multiple enzymes was downregulated by PtNPs, the treatment with CDDP affected only two TCA enzymes, indicating a different mechanism of action. Taken together, CAM assay represents and invaluable model, demonstrating the PtNPs capability of repressing angiogenesis, decrease amino acid contents and disrupt the TCA cycle.en
dc.formattextcs
dc.format.extent1-14cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMaterials Today Bio. 2023, vol. 19, issue 4, p. 1-14.en
dc.identifier.doi10.1016/j.mtbio.2023.100570cs
dc.identifier.issn2590-0064cs
dc.identifier.orcid0000-0002-8259-1566cs
dc.identifier.orcid0000-0003-4900-0020cs
dc.identifier.orcid0000-0002-7318-6470cs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.orcid0000-0002-3915-7270cs
dc.identifier.orcid0000-0003-4216-9544cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.orcid0000-0003-4122-0694cs
dc.identifier.other183016cs
dc.identifier.researcheridK-2578-2016cs
dc.identifier.researcheridH-2894-2018cs
dc.identifier.researcheridE-5702-2012cs
dc.identifier.researcheridE11072012cs
dc.identifier.researcheridD-1973-2013cs
dc.identifier.researcheridE-5711-2012cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.researcheridP-3551-2018cs
dc.identifier.scopus37080103000cs
dc.identifier.scopus14012648400cs
dc.identifier.scopus6603604023cs
dc.identifier.scopus56115133500cs
dc.identifier.urihttp://hdl.handle.net/11012/209507
dc.language.isoencs
dc.relation.ispartofMaterials Today Biocs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S2590006423000303cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2590-0064/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectPlatinum nanoparticlesen
dc.subjectCisplatinen
dc.subjectCAM assayen
dc.subjectBreast canceren
dc.subjectAmino acids metabolismen
dc.subjectTCA cycleen
dc.titleChick chorioallantoic membrane (CAM) assay for the evaluation of the antitumor and antimetastatic activity of platinum-based drugs in association with the impact on the amino acid metabolismen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-183016en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:14en
sync.item.modts2025.01.17 18:36:16en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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