Produkce polyhydroxyalkanoátů z vybraných odpadních substrátů
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Date
Authors
Vlasáková, Terézia
Advisor
Referee
Mark
A
Journal Title
Journal ISSN
Volume Title
Publisher
Vysoké učení technické v Brně. Fakulta chemická
ORCID
Abstract
Predložená bakalárska práca sa zameriava na štúdium produkcie polyhydroxyalkanoátov (PHA) baktériami Burkholderia sacchari a Burkholderia cepacia a taktiež faktorov, ktoré produkciu ovplyvňujú. Hlavnou skúmanou oblasťou bola produkcia PHA na substráte z kukuričnej siláže. V teoretickej časti bola podaná stručná charakteristika PHA, ako aj možnosti ich využitia v praxi a možnosti ich bakteriálnej produkcie kultiváciou na odpadných substrátoch. V experimentálnej časti práce bol sledovaný vplyv prekurzorov pridaných do média na nárast biomasy, produkciu PHA a inkorporáciu monoméru 3HV do polymérneho reťazca. Ďalej bola optimalizovaná metóda hydrolýzy kukuričného materiálu a na hydrolyzátoch boli kultivované baktérie. Pri kultivácii na hydrolyzátoch z kukuričného odpadu boli dosiahnuté najlepšie výsledky s použitím hydrolyzátu pripraveného kombináciou chemickej a enzymatickej hydrolýzy. Kultivácie oboch baktérii boli prevedené aj do laboratórneho bioreaktoru.
This bachelor's thesis is focused on study of polyhydroxyalkanoates (PHA) production by bacterial strains Burkholderia sacchari and Burkholderia cepacia. It also deals with influence of various factors on their production. The main studied area was PHA production from substrate prepared from corn stover. In theoretical part a brief description of PHA was given. We also summarized options on their practical applications and possibilities of their bacterial production from waste substrates. In experimental part of work the impact of used precursors on biomass growth, PHA production and incorporation of 3HV monomer into the polymer chain was monitored. Further, a method of corn material hydrolysis was optimized. Bacterial cultures were cultivated on prepared hydrolysates. The best results in cultivation on hydrolysates of corn silage were achieved when the hydrolysis was performed by combination of chemical and enzymatic way. Cultivations of both bacteria were also transferred into the laboratory bioreactor.
This bachelor's thesis is focused on study of polyhydroxyalkanoates (PHA) production by bacterial strains Burkholderia sacchari and Burkholderia cepacia. It also deals with influence of various factors on their production. The main studied area was PHA production from substrate prepared from corn stover. In theoretical part a brief description of PHA was given. We also summarized options on their practical applications and possibilities of their bacterial production from waste substrates. In experimental part of work the impact of used precursors on biomass growth, PHA production and incorporation of 3HV monomer into the polymer chain was monitored. Further, a method of corn material hydrolysis was optimized. Bacterial cultures were cultivated on prepared hydrolysates. The best results in cultivation on hydrolysates of corn silage were achieved when the hydrolysis was performed by combination of chemical and enzymatic way. Cultivations of both bacteria were also transferred into the laboratory bioreactor.
Description
Citation
VLASÁKOVÁ, T. Produkce polyhydroxyalkanoátů z vybraných odpadních substrátů [online]. Brno: Vysoké učení technické v Brně. Fakulta chemická. 2015.
Document type
Document version
Date of access to the full text
Language of document
sk
Study field
Biotechnologie
Comittee
prof. RNDr. Ivana Márová, CSc. (předseda)
prof. Ing. Peter Šimko, DrSc. (místopředseda)
doc. Ing. Jiřina Omelková, CSc. (člen)
doc. RNDr. Alena Španová, CSc. (člen)
doc. Ing. Bohuslav Rittich, CSc. (člen)
doc. Ing. Pavel Diviš, Ph.D. (člen)
Date of acceptance
2015-06-16
Defence
1. Studentka seznámila členy komise s náplní a cílem bakalářské práce.
2. Byly přečteny posudky na bakalářskou práci.
3. Studentka akceptovala všechny připomínky oponenta a na všechny otázky odpověděla v plné šíři.
Diskuse
prof. Márová: Je kukuřičná siláž nevyužitelný odpad ?
Result of defence
práce byla úspěšně obhájena