Cisplatin enhances cell stiffness and decreases invasiveness rate in prostate cancer cells by actin accumulation

Simple item page
dc.contributor.authorRaudenská, Martinacs
dc.contributor.authorKratochvílová, Monikacs
dc.contributor.authorVičar, Tomášcs
dc.contributor.authorGumulec, Jaromírcs
dc.contributor.authorBalvan, Jancs
dc.contributor.authorPolanská, Hanacs
dc.contributor.authorPřibyl, Jancs
dc.contributor.authorMasařík, Michalcs
dc.coverage.issue1cs
dc.coverage.volume9cs
dc.date.issued2019-02-07cs
dc.description.abstractWe focused on the biomechanical and morphological characteristics of prostate cancer cells and their changes resulting from the effect of docetaxel, cisplatin, and long-term zinc supplementation. Cell population surviving the treatment was characterized as follows: cell stiffness was assessed by atomic force microscopy, cell motility and invasion capacity were determined by colony forming assay, wound healing assay, coherence-controlled holographic microscopy, and real-time cell analysis. Cells of metastatic origin exhibited lower height than cells derived from the primary tumour. Cell dry mass and CAV1 gene expression followed similar trends as cell stiffness. Docetaxel- and cisplatin-surviving cells had higher stiffness, and decreased motility and invasive potential as compared to non-treated cells. This effect was not observed in zinc(II)-treated cells. We presume that cell stiffness changes may represent an important overlooked effect of cisplatin-based anti-cancer drugs. Atomic force microscopy and confocal microscopy data images used in our study are available for download in the Zenodo repository (https://zenodo.org/, Digital Object Identifiers:10.5281/zenodo.1494935).en
dc.formattextcs
dc.format.extent1-11cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationScientific Reports. 2019, vol. 9, issue 1, p. 1-11.en
dc.identifier.doi10.1038/s41598-018-38199-7cs
dc.identifier.issn2045-2322cs
dc.identifier.orcid0000-0002-9136-7873cs
dc.identifier.orcid0000-0002-9658-3444cs
dc.identifier.orcid0000-0003-1172-7195cs
dc.identifier.other158198cs
dc.identifier.researcheridC-6006-2018cs
dc.identifier.researcheridD-7638-2012cs
dc.identifier.researcheridD-9920-2012cs
dc.identifier.scopus57202426072cs
dc.identifier.scopus55769747816cs
dc.identifier.urihttp://hdl.handle.net/11012/184678
dc.language.isoencs
dc.publisherNATURE PUBLISHING GROUPcs
dc.relation.ispartofScientific Reportscs
dc.relation.urihttps://www.nature.com/articles/s41598-018-38199-7cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2045-2322/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectdocetaxelen
dc.subjectcisplatinen
dc.subjectcell stifnessen
dc.subjectzincen
dc.subjectcoherence-controlleden
dc.subjectatomic force microscopyen
dc.subjectholographic microscopyen
dc.titleCisplatin enhances cell stiffness and decreases invasiveness rate in prostate cancer cells by actin accumulationen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-158198en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:39:42en
sync.item.modts2025.01.17 16:33:45en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s41598018381997.pdf
Size:
2.78 MB
Format:
Adobe Portable Document Format
Description:
s41598018381997.pdf
Download
Collections
Ústav biomedicínského inženýrství
Chytré nanonástroje