Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow

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dc.contributor.authorGrzesiak, Jakubcs
dc.contributor.authorGawor, Jancs
dc.contributor.authorRogala, Małgorzata Martacs
dc.contributor.authorKouřilová, Xeniecs
dc.contributor.authorObruča, Stanislavcs
dc.coverage.issue3cs
dc.coverage.volume27cs
dc.date.issued2023-12-01cs
dc.description.abstractIn recent years, extremophilic microorganisms have been employed as producers of the microbial bioplastics polyhydroxyalkanoates (PHA), which are of great biotechnological value. Nevertheless, cold-loving or psychrophilic (cryophilic) bacteria have been neglected in this regard. Here, we present an investigation of the Arctic glacier-derived PHA producer Acidovorax sp. A1169. Biolog GEN III Microplates were used as a screening tool to identify the most suitable carbon substrate concerning PHA synthesis. The strain produced homopolymer poly(3-hydroxybutyrate) (PHB) most efficiently (2 g/L) at a temperature of 15 ? when supplied with fructose or mannitol as carbon sources with a substantial decrease of PHB biosynthesis at 17.5 ?. The PHB yield did not increase considerably or even decreased when carbon source concentration exceeded 10 g/L hinting that the strain is oligotrophic in nature. The strain was also capable of introducing 3-hydroxyvalerate (3HV) into the polymer structure, which is known to improve PHA thermoplastic properties. This is the first investigation providing insight into a PHA biosynthesis process by means of a true psychrophile, offering guidelines on polar-region bacteria cultivation, production of PHA and also on the methodology for genetic engineering of psychrophiles.en
dc.formattextcs
dc.format.extent1-14cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationEXTREMOPHILES. 2023, vol. 27, issue 3, p. 1-14.en
dc.identifier.doi10.1007/s00792-023-01311-5cs
dc.identifier.issn1431-0651cs
dc.identifier.orcid0000-0002-1339-6660cs
dc.identifier.orcid0000-0002-9270-195Xcs
dc.identifier.other184904cs
dc.identifier.scopus25621797900cs
dc.identifier.urihttp://hdl.handle.net/11012/245178
dc.language.isoencs
dc.publisherSpringercs
dc.relation.ispartofEXTREMOPHILEScs
dc.relation.urihttps://link.springer.com/article/10.1007/s00792-023-01311-5cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1431-0651/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectExtremophileen
dc.subjectArcticen
dc.subjectBioplasticsen
dc.subjectLow-temperatureen
dc.subjectbiotechnologyen
dc.subjectOligotrophyen
dc.titleGenetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflowen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-184904en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:38:44en
sync.item.modts2025.01.17 15:34:24en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
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