Genetic engineering of low-temperature polyhydroxyalkanoate production by Acidovorax sp. A1169, a psychrophile isolated from a subglacial outflow
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Date
2023-12-01
Authors
Grzesiak, Jakub
Gawor, Jan
Rogala, Małgorzata Marta
Kouřilová, Xenie
Obruča, Stanislav
0000-0002-1339-6660Advisor
Referee
Mark
Journal Title
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Volume Title
Publisher
Springer
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Abstract
In recent years, extremophilic microorganisms have been employed as producers of the microbial bioplastics polyhydroxyalkanoates (PHA), which are of great biotechnological value. Nevertheless, cold-loving or psychrophilic (cryophilic) bacteria have been neglected in this regard. Here, we present an investigation of the Arctic glacier-derived PHA producer Acidovorax sp. A1169. Biolog GEN III Microplates were used as a screening tool to identify the most suitable carbon substrate concerning PHA synthesis. The strain produced homopolymer poly(3-hydroxybutyrate) (PHB) most efficiently (2 g/L) at a temperature of 15 ? when supplied with fructose or mannitol as carbon sources with a substantial decrease of PHB biosynthesis at 17.5 ?. The PHB yield did not increase considerably or even decreased when carbon source concentration exceeded 10 g/L hinting that the strain is oligotrophic in nature. The strain was also capable of introducing 3-hydroxyvalerate (3HV) into the polymer structure, which is known to improve PHA thermoplastic properties. This is the first investigation providing insight into a PHA biosynthesis process by means of a true psychrophile, offering guidelines on polar-region bacteria cultivation, production of PHA and also on the methodology for genetic engineering of psychrophiles.
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Citation
EXTREMOPHILES. 2023, vol. 27, issue 3, p. 1-14.
https://link.springer.com/article/10.1007/s00792-023-01311-5
https://link.springer.com/article/10.1007/s00792-023-01311-5
Document type
Peer-reviewed
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en