Ústav informačních systémů
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- ItemIncreasing Visibility of IEC 104 Communication in the Smart Grid(BCS Learning and Development Ltd., 2019-06-25) Matoušek, Petr; Ryšavý, Ondřej; Grégr, MatějEnergy systems like smart grids are part of critical infrastructure and their interruption or blackout may have fatal consequences on energy production, distribution, and eventually the life of individual people. In order to secure communication in Industrial Control Systems (ICS) and detect cyber attacks on smart grids, we need to increase visibility of ICS communication so that an operator can see what commands are sent between ICS devices. Security monitoring of ICS transmission requires (i) retrieving monitoring data from ICS packets, (ii) processing and analyzing extracted data, (iii) visualizing the passing communication to the operator. The proposed work presents a concept of ICS flow monitoring system that extracts meta data from ICS packet headers and creates ICS flow records similarly to Netflow/IPFIX system. ICS flows represent communication in the smart grid network that is further visualized using dashboard and communication charts. Unlike traditional monitoring approach that works with network and transport layer data only, we extend flow monitoring to application layer with focus on ICS protocols. The proposed approach is demonstrated on monitoring IEC 60870-5-104 communication.
- ItemComparison of Fungal Thermophilic and Mesophilic Catalase-Peroxidases for Their Antioxidative Properties(MDPI, 2023-07-04) Poljovka, Andrej; Musil, Miloš; Bednář, David; Chovanová, Katarína; Bauerová-Hlinková, Vladena; Bellová, Jana; Kohútová, Lenka; Baráth, Peter; Zámocký, MarcelCatalase-peroxidases (KatGs) are unique bifunctional oxidoreductases that contain heme in their active centers allowing both the peroxidatic and catalatic reaction modes. These originally bacterial enzymes are broadly distributed among various fungi allowing them to cope with reactive oxygen species present in the environment or inside the cells. We used various biophysical, biochemical, and bioinformatics methods to investigate differences between catalase-peroxidases originating in thermophilic and mesophilic fungi from different habitats. Our results indicate that the architecture of the active center with a specific post-translational modification is highly similar in mesophilic and thermophilic KatG and also the peroxidatic acitivity with ABTS, guaiacol, and L-DOPA. However, only the thermophilic variant CthedisKatG reveals increased manganese peroxidase activity at elevated temperatures. The catalatic activity releasing molecular oxygen is comparable between CthedisKatG and mesophilic MagKatG1 over a broad temperature range. Two constructed point mutations in the active center were performed selectively blocking the formation of described post-translational modification in the active center. They exhibited a total loss of catalatic activity and changes in the peroxidatic activity. Our results indicate the capacity of bifunctional heme enzymes in the variable reactivity for potential biotech applications.
- ItemSatellite DNA and Transposable Elements in Seabuckthorn ( Hippophae rhamnoides ), a Dioecious Plant with Small Y and Large X Chromosomes(2017-02-02) Puterová, Janka; Razumova, Olga; Martínek, Tomáš; Alexandrov, Oleg; Divashuk, Mikhail; Kubát, Zdeněk; Hobza, Roman; Karlov, Gennady; Kejnovský, EduardSeabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome.We carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and 45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration. Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes.
- ItemResponsible And Safe Home Metering: How to Design A Privacy-Friendly Metering System?(IGI Global, 2023-04-03) Polčák, LiborThe European directive on energy efficiency requires that all meters in multi-apartment buildings installed after 25 October 2020 shall be remotely readable devices when technically feasible and cost-effective in terms of being proportionate to the potential energy savings. The European Commission Recommendation of 9 March 2012 on preparations for the roll-out of smart metering systems (2012/148/EU) explicitly mentions that smart metering predominantly processes personal data. This chapter recommends how to design a metering system that fully conforms to legal regulations. The main contribution is the recommendation of eight steps for data controllers that make metering systems legally compliant. Additionally, the chapter lists recommendations for smart meter manufacturers that remove the burden of being a controller of the processing. The recommendations apply to the distribution of electricity, water, gas, heat, cooling, and other energies. The chapter shows that the recommendations can be generalized for smart home deployments.
- ItemMetering Homes: Do Energy Efficiency and Privacy Need to be in Conflict?(SciTePress - Science and Technology Publications, 2022-07-11) Polčák, Libor; Matoušek, PetrThe European directive on energy efficiency requires that all meters in multi-apartment buildings installed after 25 October 2020 shall be remotely readable devices where technically feasible and cost effective in terms of being proportionate in relation to the potential energy savings. In practise, this means that some manufacturers produce meters that monitor energy consumption in very short intervals, for example, less than two minutes; even though the directive expects to provide billing information to consumers only once a month starting from 2022. This paper reviews privacy and security risks stemming from the short readouts and provides recommendations for manufacturers and suppliers. The paper focuses on a Wireless M-Bus metering devices that we observed being sold and advertised as the solution to fulfill the European directive on energy efficiency requirements. Nevertheless, we believe that many recommendations and observations are applicable also to other protocols.