Characterization of Microbiota Composition and Presence of Selected Antibiotic Resistance Genes in Carriage Water of Ornamental Fish

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dc.contributor.authorGeržová, Lenkacs
dc.contributor.authorVídeňská, Petracs
dc.contributor.authorFaldynová, Marcelacs
dc.contributor.authorSedlář, Karelcs
dc.contributor.authorProvazník, Valentýnacs
dc.contributor.authorČížek, Aloiscs
dc.contributor.authorRychlík, Ivancs
dc.coverage.issue8cs
dc.coverage.volume9cs
dc.date.issued2014-08-01cs
dc.description.abstractInternational trade with ornamental fish is gradually recognized as an important source of a wide range of different antibiotic resistant bacteria. In this study we therefore characterized the prevalence of selected antibiotic resistance genes in the microbiota found in the carriage water of ornamental fish originating from 3 different continents. Real-time PCR quantification showed that the sul1 gene was present in 11 out of 100 bacteria. tet(A) was present in 6 out of 100 bacteria and strA, tet(G), sul2 and aadA were present in 1–2 copies per 100 bacteria. Class I integrons were quite common in carriage water microbiota, however, pyrosequencing showed that only 12 different antibiotic gene cassettes were present in class I integrons. The microbiota characterized by pyrosequencing of the V3/V4 variable region of 16S rRNA genes consisted of Proteobacteria (48%), Bacteroidetes (29.5%), Firmicutes (17.8%), Actinobacteria (2.1%) and Fusobacteria (1.6%). Correlation analysis between antibiotic resistance gene prevalence and microbiota composition verified by bacterial culture showed that major reservoirs of sul1 sul2, tet(A), tet(B) tet(G), cat, cml, bla, strA, aacA, aph and aadA could be found among Alpha-, Beta- and Gammaproteobacteria with representatives of Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae and Comamonadaceae being those most positively associated with the tested antibiotic resistance genes.en
dc.description.abstractMezinárodní obchod s okrasnými rybami je významný zdroj velkého množství antibioticky rezistenstních bakterií. V této studii jsme charakterizovali vybrané geny antibiotické rezistence v mikroflóře z vody použité pro převoz okrasných ryb ze tří různých kontinentů. Reali-time PCR analýza ukázala především velkou abundanci genů sul1 a tet(A).cs
dc.formattextcs
dc.format.extente103865-e103865cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPLOS ONE. 2014, vol. 9, issue 8, p. e103865-e103865.en
dc.identifier.doi10.1371/journal.pone.0103865cs
dc.identifier.issn1932-6203cs
dc.identifier.orcid0000-0002-8269-4020cs
dc.identifier.orcid0000-0002-3422-7938cs
dc.identifier.other108700cs
dc.identifier.researcheridK-1120-2014cs
dc.identifier.researcheridF-4121-2012cs
dc.identifier.scopus56309904900cs
dc.identifier.scopus6701729526cs
dc.identifier.urihttp://hdl.handle.net/11012/69203
dc.language.isoencs
dc.publisherPublic Library of Sciencecs
dc.relation.ispartofPLOS ONEcs
dc.relation.urihttp://europepmc.org/articles/PMC4118911?pdf=rendercs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1932-6203/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectmetagenomicsen
dc.subjectbacteriaen
dc.subjectmicrobiotaen
dc.subjectfishen
dc.subjectmetagenomika
dc.subjectbakterie
dc.subjectmikroflóra
dc.subjectryby
dc.titleCharacterization of Microbiota Composition and Presence of Selected Antibiotic Resistance Genes in Carriage Water of Ornamental Fishen
dc.title.alternativeCharakterizace skladby mikroflóry a přítomnosti vybraných genů antibiotické rezistence ve vodách pro převoz okrasných rybcs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-108700en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:40:04en
sync.item.modts2025.01.17 15:12:34en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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