NANOBLAST: Python Tool for Raw Nanopore Signal Processing

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but.event.date23.04.2024cs
but.event.titleSTUDENT EEICT 2024cs
dc.contributor.authorSuriak, Martin
dc.contributor.authorNykrýnová, Markéta
dc.date.accessioned2024-07-09T07:38:39Z
dc.date.available2024-07-09T07:38:39Z
dc.date.issued2024cs
dc.description.abstractOxford Nanopore Technologies’ sequencers enable direct real-time DNA/RNA sequencing. While numerous tools aid in analyzing the nanopore output data, offering functions such as visualizing raw signals with highlighted nucleotide positions, none provide a complete solution for exporting analyzed data into a clear, comprehensive file. In response, a Python tool has been developed to streamline various tasks. This includes searching for specific nucleotide sequences using BLAST, plotting raw signals with detected nucleotide bases, and generating a comprehensive file containing all essential information. The tool integrates components for handling raw nanopore data, extracting crucial information from the basecalling process using SAM file handlers, and utilizing a BLAST search engine. Employing a comprehensive algorithm, it can handle both the old FAST5 and the novel POD5 formats, enabling the identification of any nucleotide sequence and its corresponding signal.en
dc.formattextcs
dc.format.extent24-27cs
dc.format.mimetypeapplication/pdfen
dc.identifier.citationProceedings I of the 30st Conference STUDENT EEICT 2024: General papers. s. 24-27. ISBN 978-80-214-6231-1cs
dc.identifier.isbn978-80-214-6231-1
dc.identifier.issn2788-1334
dc.identifier.urihttps://hdl.handle.net/11012/249234
dc.language.isoencs
dc.publisherVysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologiícs
dc.relation.ispartofProceedings I of the 30st Conference STUDENT EEICT 2024: General papersen
dc.relation.urihttps://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_1.pdfcs
dc.rights© Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologiícs
dc.rights.accessopenAccessen
dc.subjectsquiggleen
dc.subjectsequencingen
dc.subjectsignal processingen
dc.titleNANOBLAST: Python Tool for Raw Nanopore Signal Processingen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
eprints.affiliatedInstitution.departmentFakulta elektrotechniky a komunikačních technologiícs
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