Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells

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dc.contributor.authorBáčová, Janacs
dc.contributor.authorKnotek, Petrcs
dc.contributor.authorKopecká, Kateřinacs
dc.contributor.authorHromádko, Luděkcs
dc.contributor.authorČapek, Jancs
dc.contributor.authorNývltová, Pavlínacs
dc.contributor.authorBrůčková, Lenkacs
dc.contributor.authorSchröterová, Ladislavacs
dc.contributor.authorŠestáková, Blankacs
dc.contributor.authorPalarčík, Jiřícs
dc.contributor.authorMotola, Martincs
dc.contributor.authorČížková, Danacs
dc.contributor.authorBezrouk, Alešcs
dc.contributor.authorHandl, Jiřícs
dc.contributor.authorFiala, Zdeněkcs
dc.contributor.authorRudolf, Emilcs
dc.contributor.authorBílková, Zuzanacs
dc.contributor.authorMacák, Jancs
dc.contributor.authorRoušar, Tomášcs
dc.coverage.issue1cs
dc.coverage.volume17cs
dc.date.accessioned2022-12-12T15:56:15Z
dc.date.available2022-12-12T15:56:15Z
dc.date.issued2022-09-30cs
dc.description.abstractPurpose: Titanium dioxide nanoparticles, 25 nm in size of crystallites (TiO2 P25), are among the most produced nanomaterials worldwide. The broad use of TiO2 P25 in material science has implied a request to evaluate their biological effects, especially in the lungs. Hence, the pulmonary A549 cell line has been used to estimate the effects of TiO2 P25. However, the reports have provided dissimilar results on caused toxicity. Surprisingly, the physicochemical factors influencing TiO2 P25 action in biological models have not been evaluated in most reports. Thus, the objective of the present study is to characterize the preparation of TiO2 P25 for biological testing in A549 cells and to evaluate their biological effects.Methods: We determined the size and crystallinity of TiO2 P25. We used four techniques for TiO2 P25 dispersion. We estimated the colloid stability of TiO2 P25 in distilled water, isotonic NaCl solution, and cell culture medium. We applied the optimal dispersion conditions for testing the biological effects of TiO2 P25 (0-100 mu g.mL-1) in A549 cells using biochemical assays (dehydrogenase activity, glutathione levels) and microscopy.Results: We found that the use of fetal bovine serum in culture medium is essential to maintain sufficient colloid stability of dispersed TiO2 P25. Under these conditions, TiO2 P25 were unable to induce a significant impairment of A549 cells according to the results of biochemical and microscopy evaluations. When the defined parameters for the use of TiO2 P25 in A549 cells were met, similar results on the biological effects of TiO2 P25 were obtained in two independent cell laboratories.Conclusion: We optimized the experimental conditions of TiO2 P25 preparation for toxicity testing in A549 cells. The results presented here on TiO2 P25-induced cellular effects are reproducible. Therefore, our results can be helpful for other researchers using TiO2 P25 as a reference material.en
dc.formattextcs
dc.format.extent4211-4225cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Nanomedicine. 2022, vol. 17, issue 1, p. 4211-4225.en
dc.identifier.doi10.2147/IJN.S374955cs
dc.identifier.issn1178-2013cs
dc.identifier.other179712cs
dc.identifier.urihttp://hdl.handle.net/11012/208681
dc.language.isoencs
dc.publisherDove Presscs
dc.relation.ispartofInternational Journal of Nanomedicinecs
dc.relation.urihttps://www.dovepress.com/evaluating-the-use-of-tio2-nanoparticles-for-toxicity-testing-in-pulmo-peer-reviewed-fulltext-article-IJNcs
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unportedcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1178-2013/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/cs
dc.subjecttitanium dioxideen
dc.subjectnanoparticlesen
dc.subjectP25en
dc.subjectnanotoxicityen
dc.subjectA549 cellsen
dc.subjectdispersionen
dc.titleEvaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cellsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-179712en
sync.item.dbtypeVAVen
sync.item.insts2022.12.12 16:56:15en
sync.item.modts2022.12.12 16:14:35en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé nízkodimenzionální nanomateriálycs
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