Phenotypic and genomic analysis of isopropanol and 1,3-propanediol producer Clostridium diolis DSM 15410

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dc.contributor.authorSedlář, Karelcs
dc.contributor.authorVasylkivska, Marynacs
dc.contributor.authorMusilová, Janacs
dc.contributor.authorBranská, Barboracs
dc.contributor.authorProvazník, Ivocs
dc.contributor.authorPatáková, Petracs
dc.coverage.issue1cs
dc.coverage.volume113cs
dc.date.accessioned2021-03-23T11:53:52Z
dc.date.available2021-03-23T11:53:52Z
dc.date.issued2020-11-07cs
dc.description.abstractClostridium diolis DSM 15410 is a type strain of solventogenic clostridium capable of conducting isopropanol-butanol-ethanol fermentation. By studying its growth on different carbohydrates, we verified its ability to utilize glycerol and produce 1,3-propanediol and discovered its ability to produced isopropanol. Complete genome sequencing showed that its genome is a single circular chromosome and belongs to the cluster I (sensu scricto) of the genus Clostridium. By cultivation analysis we highlighted its specific behavior in comparison to two selected closely related strains. Despite the fact that several CRISPR loci were found, 16 putative prophages showed the ability to receive foreign DNA. Thus, the strain has the necessary features for future engineering of its 1,3-propanediol biosynthetic pathway and for the possible industrial utilization in the production of biofuels.en
dc.description.embargo2021-11-08cs
dc.formattextcs
dc.format.extent1109-1119cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationGENOMICS. 2020, vol. 113, issue 1, p. 1109-1119.en
dc.identifier.doi10.1016/j.ygeno.2020.11.007cs
dc.identifier.issn0888-7543cs
dc.identifier.other165852cs
dc.identifier.urihttp://hdl.handle.net/11012/196477
dc.language.isoencs
dc.publisherElseviercs
dc.relation.ispartofGENOMICScs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S088875432032005Xcs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0888-7543/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectClostridium diolis; complete genome; solventogenic; IBE; 1en
dc.subject3-propanediolen
dc.titlePhenotypic and genomic analysis of isopropanol and 1,3-propanediol producer Clostridium diolis DSM 15410en
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionacceptedVersionen
sync.item.dbidVAV-165852en
sync.item.dbtypeVAVen
sync.item.insts2021.11.08 00:54:51en
sync.item.modts2021.11.08 00:16:46en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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