Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level

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dc.contributor.authorPatáková, Petracs
dc.contributor.authorBranská, Barboracs
dc.contributor.authorSedlář, Karelcs
dc.contributor.authorVasylkivska, Marynacs
dc.contributor.authorŠabatová, Kateřinacs
dc.contributor.authorKolek, Jancs
dc.contributor.authorCicková, Pavlínacs
dc.contributor.authorProvazník, Valentýnacs
dc.coverage.issue1cs
dc.coverage.volume9cs
dc.date.issued2019-02-04cs
dc.description.abstractClostridium beijerinckii NRRL B-598 is a sporulating, butanol and hydrogen producing strain that utilizes carbohydrates by the acetone-butanol-ethanol (ABE) fermentative pathway. The pathway consists of two metabolic phases, acidogenesis and solventogenesis, from which the latter one can be coupled with sporulation. Thorough transcriptomic profiling during a complete life cycle and both metabolic phases completed with flow cytometry, microscopy and a metabolites analysis helped to find out key genes involved in particular cellular events. The description of genes/operons that are closely involved in metabolism or the cell cycle is a necessary condition for metabolic engineering of the strain and will be valuable for all C. beijerinckii strains and other Clostridial species. The study focused on glucose transport and catabolism, hydrogen formation, metabolic stress response, binary fission, motility/chemotaxis and sporulation, which resulted in the composition of the unique image reflecting clostridial population changes. Surprisingly, the main change in expression of individual genes was coupled with the sporulation start and not with the transition from acidogenic to solventogenic metabolism. As expected, solvents formation started at pH decrease and the accumulation of butyric and acetic acids in the cultivation medium.en
dc.formattextcs
dc.format.extent1-21cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationScientific Reports. 2019, vol. 9, issue 1, p. 1-21.en
dc.identifier.doi10.1038/s41598-018-37679-0cs
dc.identifier.issn2045-2322cs
dc.identifier.orcid0000-0002-8269-4020cs
dc.identifier.orcid0000-0001-5143-9521cs
dc.identifier.orcid0000-0003-1341-7975cs
dc.identifier.orcid0000-0002-3422-7938cs
dc.identifier.other155993cs
dc.identifier.researcheridK-1120-2014cs
dc.identifier.researcheridAAC-2736-2019cs
dc.identifier.researcheridF-4121-2012cs
dc.identifier.scopus56309904900cs
dc.identifier.scopus57205673155cs
dc.identifier.scopus6701729526cs
dc.identifier.urihttp://hdl.handle.net/11012/179544
dc.language.isoencs
dc.publisherSpringer Naturecs
dc.relation.ispartofScientific Reportscs
dc.relation.urihttp://link.springer.com/article/10.1038/s41598-018-37679-0cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2045-2322/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectClostridium beijerinckiien
dc.subjectABE fermentationen
dc.subjecttranscriptomic profilingen
dc.subjectlife cycleen
dc.subjectsporulationen
dc.titleAcidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic levelen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-155993en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:39:41en
sync.item.modts2025.01.17 16:37:00en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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