Antimicrobial Activity of Gelatin Nanofibers Enriched by Essential Oils against Cutibacterium acnes and Staphylococcus epidermidis

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dc.contributor.authorUhlířová, Renatacs
dc.contributor.authorLangová, Denisacs
dc.contributor.authorBendová, Agátacs
dc.contributor.authorGross, Michalcs
dc.contributor.authorSkoumalová, Petracs
dc.contributor.authorMárová, Ivanacs
dc.coverage.issue5cs
dc.coverage.volume13cs
dc.date.issued2023-03-22cs
dc.description.abstractAcne vulgaris is a prevalent skin condition that is caused by an imbalance in skin microbiomes mainly by the overgrowth of strains such as Cutibacterium acnes and Staphylococcus epidermidis which affect both teenagers and adults. Drug resistance, dosing, mood alteration, and other issues hinder traditional therapy. This study aimed to create a novel dissolvable nanofiber patch containing essential oils (EOs) from Lavandula angustifolia and Mentha piperita for acne vulgaris treatment. The EOs were characterized based on antioxidant activity and chemical composition using HPLC and GC/MS analysis. The antimicrobial activity against C. acnes and S. epidermidis was observed by the determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The MICs were in the range of 5.7-9.4 mu L/mL, and MBCs 9.4-25.0 mu L/mL. The EOs were integrated into gelatin nanofibers by electrospinning and SEM images of the fibers were taken. Only the addition of 20% of pure essential oil led to minor diameter and morphology alteration. The agar diffusion tests were performed. Pure and diluted Eos in almond oil exhibited a strong antibacterial effect on C. acnes and S. epidermidis. After incorporation into nanofibers, we were able to focus the antimicrobial effect only on the spot of application with no effect on the surrounding microorganisms. Lastly, for cytotoxicity evaluation, and MTT assay was performed with promising results that samples in the tested range had a low impact on HaCaT cell line viability. In conclusion, our gelatin nanofibers containing EOs are suitable for further investigation as prospective antimicrobial patches for acne vulgaris local treatment.en
dc.description.abstractAcne vulgaris is a prevalent skin condition that is caused by an imbalance in skin microbiomes mainly by the overgrowth of strains such as Cutibacterium acnes and Staphylococcus epidermidis which affect both teenagers and adults. Drug resistance, dosing, mood alteration, and other issues hinder traditional therapy. This study aimed to create a novel dissolvable nanofiber patch containing essential oils (EOs) from Lavandula angustifolia and Mentha piperita for acne vulgaris treatment. The EOs were characterized based on antioxidant activity and chemical composition using HPLC and GC/MS analysis. The antimicrobial activity against C. acnes and S. epidermidis was observed by the determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The MICs were in the range of 5.7-9.4 mu L/mL, and MBCs 9.4-25.0 mu L/mL. The EOs were integrated into gelatin nanofibers by electrospinning and SEM images of the fibers were taken. Only the addition of 20% of pure essential oil led to minor diameter and morphology alteration. The agar diffusion tests were performed. Pure and diluted Eos in almond oil exhibited a strong antibacterial effect on C. acnes and S. epidermidis. After incorporation into nanofibers, we were able to focus the antimicrobial effect only on the spot of application with no effect on the surrounding microorganisms. Lastly, for cytotoxicity evaluation, and MTT assay was performed with promising results that samples in the tested range had a low impact on HaCaT cell line viability. In conclusion, our gelatin nanofibers containing EOs are suitable for further investigation as prospective antimicrobial patches for acne vulgaris local treatment.cs
dc.formattextcs
dc.format.extent1-18cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationNanomaterials. 2023, vol. 13, issue 5, p. 1-18.en
dc.identifier.doi10.3390/nano13050844cs
dc.identifier.issn2079-4991cs
dc.identifier.orcid0000-0001-5912-7928cs
dc.identifier.orcid0000-0002-2929-8491cs
dc.identifier.orcid0000-0003-3514-1260cs
dc.identifier.orcid0000-0003-4705-7752cs
dc.identifier.orcid0000-0002-2095-1837cs
dc.identifier.other185011cs
dc.identifier.researcheridA-6994-2016cs
dc.identifier.researcheridA-6897-2016cs
dc.identifier.scopus56477418300cs
dc.identifier.scopus6603465110cs
dc.identifier.urihttp://hdl.handle.net/11012/244277
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofNanomaterialscs
dc.relation.urihttps://www.mdpi.com/2079-4991/13/5/844cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2079-4991/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectnanofibersen
dc.subjectantimicrobial activityen
dc.subjectacne vulgarisen
dc.subjectessential oilsen
dc.subjectLavandula angustifoliaen
dc.subjectMentha piperitaen
dc.subjectlocal treatmenten
dc.titleAntimicrobial Activity of Gelatin Nanofibers Enriched by Essential Oils against Cutibacterium acnes and Staphylococcus epidermidisen
dc.title.alternativeAntimicrobiální aktivita želatinových nanovláken obohacených esenciálními oleji proti Cutibacterium acnes a Staphylococcus epidermidis.cs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-185011en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:38:42en
sync.item.modts2025.01.17 16:47:54en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
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