Effect of Silk Fibroin on the Mechanical and Transport Properties of Agarose Hydrogels

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dc.contributor.authorRichterová, Veronikacs
dc.contributor.authorPekař, Miloslavcs
dc.coverage.issue10cs
dc.coverage.volume10cs
dc.date.accessioned2025-04-04T11:56:20Z
dc.date.available2025-04-04T11:56:20Z
dc.date.issued2024-09-24cs
dc.description.abstractIn this work, the effect of incorporating silk fibroin, a fibrous biocompatible protein, into physically cross-linked agarose hydrogels was investigated as a simple model study to examine how supramolecular fibrous structures influence the properties of the hydrogels. The rheological and transport properties were studied. Fibroin did not change the general viscoelastic properties of the investigated hydrogels but changed the viscoelastic moduli values and also the mesh size, as calculated from rheometry data. Fibroin influenced the mechanical properties depending on its concentration: at lower concentrations, it increased the mesh size, while at higher concentrations, it acted as a filler, decreasing the mesh size. Similarly, the storage and loss moduli were affected, either increasing or decreasing based on the fibroin concentration. The fibroin effect on the diffusion of two dyes differing in their charge was the result of a combination of structural effects, responsible also for changes in the rheological properties, and a result of electrostatic interactions between the charged groups. For positively charged methylene blue, low fibroin concentrations accelerated diffusion, while higher concentrations slowed it by filling network vacancies. In contrast, for negatively charged eosin-B, fibroin strongly impeded diffusion at all concentrations due to electrostatic repulsion, leading to its accumulation at the hydrogel interface. The findings of this work may contribute to an understanding of the behavior of the extracellular matrix or soft tissues as well as to the development of the tailored design of hydrogel materials.en
dc.formattextcs
dc.format.extent1-15cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationGels. 2024, vol. 10, issue 10, p. 1-15.en
dc.identifier.doi10.3390/gels10100611cs
dc.identifier.issn2310-2861cs
dc.identifier.orcid0000-0003-0191-8991cs
dc.identifier.orcid0000-0002-3878-0917cs
dc.identifier.other189720cs
dc.identifier.urihttps://hdl.handle.net/11012/250726
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofGelscs
dc.relation.urihttps://www.mdpi.com/2310-2861/10/10/611cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2310-2861/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectfibroinen
dc.subjectagaroseen
dc.subjecthydrogelen
dc.subjectrheologyen
dc.subjectmesh sizeen
dc.subjectdiffusionen
dc.titleEffect of Silk Fibroin on the Mechanical and Transport Properties of Agarose Hydrogelsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-189720en
sync.item.dbtypeVAVen
sync.item.insts2025.04.04 13:56:20en
sync.item.modts2025.04.02 09:32:05en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav fyzikální a spotřební chemiecs
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