Long-term zinc treatment alters the mechanical properties and metabolism of prostate cancer cells

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dc.contributor.authorNavrátil, Jiřícs
dc.contributor.authorKratochvílová, Monikacs
dc.contributor.authorRaudenská, Martinacs
dc.contributor.authorBalvan, Jancs
dc.contributor.authorVičar, Tomášcs
dc.contributor.authorPetrláková, Kateřinacs
dc.contributor.authorSuzuki, Kanakocs
dc.contributor.authorJadrná, Luciecs
dc.contributor.authorBurša, Jiřícs
dc.contributor.authorKräter, Martincs
dc.contributor.authorKyoohyun, Kimcs
dc.contributor.authorMasařík, Michalcs
dc.contributor.authorGumulec, Jaromírcs
dc.coverage.issue1cs
dc.coverage.volume24cs
dc.date.accessioned2025-04-04T11:56:47Z
dc.date.available2025-04-04T11:56:47Z
dc.date.issued2024-10-11cs
dc.description.abstractThe failure of intracellular zinc accumulation is a key process in prostate carcinogenesis. Although prostate cancer cells can accumulate zinc after long-term exposure, chronic zinc oversupply may accelerate prostate carcinogenesis or chemoresistance. Because cancer progression is associated with energetically demanding cytoskeletal rearrangements, we investigated the effect of long-term zinc presence on biophysical parameters, ATP production, and EMT characteristics of two prostate cancer cell lines (PC-3, 22Rv1). Prolonged exposure to zinc increased ATP production, spare respiratory capacity, and induced a response in PC-3 cells, characterized by remodeling of vimentin and a shift of cell dry mass density and caveolin-1 to the perinuclear region. This zinc-induced remodeling correlated with a greater tendency to maintain actin architecture despite inhibition of actin polymerization by cytochalasin. Zinc partially restored epithelial characteristics in PC-3 cells by decreasing vimentin expression and increasing E-cadherin. Nevertheless, the expression of E-cadherin remained lower than that observed in predominantly oxidative, low-invasive 22Rv1 cells. Following long-term zinc exposure, we observed an increase in cell stiffness associated with an increased refractive index in the perinuclear region and an increased mitochondrial content. The findings of the computational simulations indicate that the mechanical response cannot be attributed exclusively to alterations in cytoskeletal composition. This observation suggests the potential involvement of an additional, as yet unidentified, mechanical contributor. These findings indicate that long-term zinc exposure alters a group of cellular parameters towards an invasive phenotype, including an increase in mitochondrial number, ATP production, and cytochalasin resistance. Ultimately, these alterations are manifested in the biomechanical properties of the cells.en
dc.formattextcs
dc.format.extent313-cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationCancer Cell International. 2024, vol. 24, issue 1, p. 313-.en
dc.identifier.doi10.1186/s12935-024-03495-ycs
dc.identifier.issn1475-2867cs
dc.identifier.orcid0000-0002-9136-7873cs
dc.identifier.orcid0009-0003-9680-3595cs
dc.identifier.orcid0000-0002-2960-185Xcs
dc.identifier.orcid0000-0003-1172-7195cs
dc.identifier.orcid0000-0002-9658-3444cs
dc.identifier.other191294cs
dc.identifier.researcheridC-6006-2018cs
dc.identifier.researcheridMHQ-8717-2025cs
dc.identifier.researcheridH-7463-2016cs
dc.identifier.researcheridD-9920-2012cs
dc.identifier.researcheridD-7638-2012cs
dc.identifier.scopus57202426072cs
dc.identifier.scopus59321264600cs
dc.identifier.scopus6602676789cs
dc.identifier.scopus55769747816cs
dc.identifier.urihttps://hdl.handle.net/11012/250784
dc.language.isoencs
dc.publisherBMCcs
dc.relation.ispartofCancer Cell Internationalcs
dc.relation.urihttps://cancerci.biomedcentral.com/articles/10.1186/s12935-024-03495-ycs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1475-2867/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectMechanobiologyen
dc.subjectZincen
dc.subjectActinen
dc.subjectCytoskeletonen
dc.subjectMitochondriaen
dc.subjectVimentinen
dc.subjectMetabolismen
dc.subjectCanceren
dc.titleLong-term zinc treatment alters the mechanical properties and metabolism of prostate cancer cellsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-191294en
sync.item.dbtypeVAVen
sync.item.insts2025.04.04 13:56:47en
sync.item.modts2025.04.03 15:32:06en
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav mechaniky těles, mechatroniky a biomechanikycs
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