Determination of Renal Distribution of Zinc, Copper, Iron, and Platinum in Mouse Kidney Using LA-ICP-MS

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dc.contributor.authorŠtěpka, Petrcs
dc.contributor.authorKratochvílová, Monikacs
dc.contributor.authorKuchynka, Michaelacs
dc.contributor.authorRaudenská, Martinacs
dc.contributor.authorHolcova Polanska, Hanacs
dc.contributor.authorVičar, Tomášcs
dc.contributor.authorVaculovic, Tomascs
dc.contributor.authorVaculovičová, Markétacs
dc.contributor.authorMasařík, Michalcs
dc.coverage.issue1cs
dc.coverage.volume2021cs
dc.date.issued2021-10-26cs
dc.description.abstractThe main dose-limiting side effect of cisplatin is nephrotoxicity. The utilization of cisplatin is an issue of balancing tumour toxicity versus platinum-induced nephrotoxicity. In this study, we focused on intraorgan distribution of common essential trace elements zinc, copper, and iron in healthy mouse kidneys and distribution of platinum after cisplatin treatment. Renal distribution in 12 nontreated Nu-Nu mice (males) was assessed by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Furthermore, 9 Nu-Nu mice were treated with cisplatin. The order of elements concentration in kidneys was as follows: Fe>Zn>Cu. All three metals showed the higher concentrations at the cortex and medulla (28.60, 3.35, and 93.83g/g for Zn, Cu, and Fe, respectively) and lower concentration at the pelvis and the urinary tract (20.20, 1.93, and 62.48g/g for Zn, Cu, and Fe, respectively). No statistically significant difference between cortex and medulla was observed for these elements. After platinum treatment, the concentration of platinum in kidneys was enhanced more than 60-times, . Platinum significantly showed the highest accumulation in cortex (2.11g/g) with a gradient distribution. Platinum was less accumulated in medulla and pelvis than in cortex, and the lowest accumulation occurred in the urinary tract (1.13g/g). Image processing has been successfully utilized to colocalize metal distribution using LA-ICP-MS and histological samples images.en
dc.formattextcs
dc.format.extent1-8cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationBioMed Research International. 2021, vol. 2021, issue 1, p. 1-8.en
dc.identifier.doi10.1155/2021/6800294cs
dc.identifier.issn2314-6141cs
dc.identifier.orcid0000-0002-1686-4423cs
dc.identifier.orcid0000-0002-9136-7873cs
dc.identifier.orcid0000-0002-6771-1304cs
dc.identifier.orcid0000-0003-1172-7195cs
dc.identifier.other173252cs
dc.identifier.researcheridABE-5364-2021cs
dc.identifier.researcheridC-6006-2018cs
dc.identifier.researcheridE-5583-2016cs
dc.identifier.researcheridD-9920-2012cs
dc.identifier.scopus53464444600cs
dc.identifier.scopus57202426072cs
dc.identifier.urihttp://hdl.handle.net/11012/203062
dc.language.isoencs
dc.publisherHindawics
dc.relation.ispartofBioMed Research Internationalcs
dc.relation.urihttps://www.hindawi.com/journals/bmri/2021/6800294/cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2314-6141/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectwithout keyworden
dc.titleDetermination of Renal Distribution of Zinc, Copper, Iron, and Platinum in Mouse Kidney Using LA-ICP-MSen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-173252en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:39:51en
sync.item.modts2025.01.17 15:25:47en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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