Diffusion Behavior of Analyte Molecules in a Nanoporous Matrix Created from Polystyrene Nanoparticles
Loading...
Date
2023
Authors
Košelová, Zuzana
ORCID
Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Altmetrics
Abstract
This study investigates the diffusion behavior ofanalyte molecules in a nanoporous matrix of polystyrene particles.Two approaches, steady-state current and potential stepchronoamperometry were employed to calculate diffusioncoefficients and compare the effect of nanoporous matrices withdifferent diameter of pores and different method ofimmobilization of enzyme. Pores were created with close packingof polystyrene nanoparticles. The covalent and cross-linkingimmobilization were used to immobilize the enzyme.Chronoamperometric measurement was applied to detect analyte.The study found that the presence of nanoporous matricesimmobilized on p-lysine affects diffusion. The potential stepchronoamperometry method showed the improvement ofdiffusion. This study provides insight into the diffusion behaviorof analyte molecules in nanoporous matrices, which can be usefulin optimizing the design of amperometric biosensors.
Description
Citation
Proceedings II of the 29st Conference STUDENT EEICT 2023: Selected papers. s. 248-252. ISBN 978-80-214-6154-3
https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2023_sbornik_2_v2.pdf
https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2023_sbornik_2_v2.pdf
Document type
Peer-reviewed
Document version
Published version
Date of access to the full text
Language of document
en
Study field
Comittee
Date of acceptance
Defence
Result of defence
Document licence
© Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií