Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion

Simple item page
dc.contributor.authorTolde, Ondřejcs
dc.contributor.authorGandalovičová, Anetacs
dc.contributor.authorKřížová, Anetacs
dc.contributor.authorVeselý, Pavelcs
dc.contributor.authorChmelík, Radimcs
dc.contributor.authorRösel, Danielcs
dc.contributor.authorBrábek, Jancs
dc.coverage.issue8cs
dc.date.issued2018-08-13cs
dc.description.abstractObservation and analysis of cancer cell behaviour in 3D environment is essential for full understanding of the mechanisms of cancer cell invasion. However, label-free imaging of live cells in 3D conditions is optically more challenging than in 2D. Quantitative phase imaging provided by coherence controlled holographic microscopy produces images with enhanced information compared to ordinary light microscopy and, due to inherent coherence gate effect, enables observation of live cancer cells’ activity even in scattering milieu such as the 3D collagen matrix. Exploiting the dynamic phase differences method, we for the first time describe dynamics of differences in cell mass distribution in 3D migrating mesenchymal and amoeboid cancer cells, and also demonstrate that certain features are shared by both invasion modes. We found that amoeboid fibrosarcoma cells’ membrane blebbing is enhanced upon constriction and is also occasionally present in mesenchymally invading cells around constricted nuclei. Further, we demonstrate that both leading protrusions and leading pseudopods of invading fibrosarcoma cells are defined by higher cell mass density. In addition, we directly document bundling of collagen fibres by protrusions of mesenchymal fibrosarcoma cells. Thus, such a non-invasive microscopy offers a novel insight into cellular events during 3D invasion.en
dc.formattextcs
dc.format.extent1-13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationScientific Reports. 2018, issue 8, p. 1-13.en
dc.identifier.doi10.1038/s41598-018-30408-7cs
dc.identifier.issn2045-2322cs
dc.identifier.orcid0000-0002-9919-6204cs
dc.identifier.orcid0000-0003-3420-395Xcs
dc.identifier.orcid0000-0001-5410-4794cs
dc.identifier.other149217cs
dc.identifier.researcheridG-8475-2014cs
dc.identifier.researcheridD-9921-2012cs
dc.identifier.researcheridD-7616-2012cs
dc.identifier.scopus6603192372cs
dc.identifier.urihttp://hdl.handle.net/11012/84157
dc.language.isoencs
dc.publisherSpringer Naturecs
dc.relation.ispartofScientific Reportscs
dc.relation.urihttp://link.springer.com/article/10.1038/s41598-018-30408-7cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2045-2322/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectQuantitative phase imagingen
dc.subjectholographic microscopyen
dc.subjectcancer cell invasionen
dc.titleQuantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasionen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-149217en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:48:31en
sync.item.modts2025.01.17 15:17:10en
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav fyzikálního inženýrstvícs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Experimentální biofotonikacs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s41598018304087.pdf
Size:
2.61 MB
Format:
Adobe Portable Document Format
Description:
s41598018304087.pdf
Download
Collections
Experimentální biofotonika
Ústav fyzikálního inženýrství