Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation

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dc.contributor.authorVojtová, Lucycs
dc.contributor.authorPavliňáková, Veronikacs
dc.contributor.authorMuchová, Johanacs
dc.contributor.authorVerčimáková, Katarínacs
dc.contributor.authorBrtníková, Janacs
dc.contributor.authorKnoz, Martincs
dc.contributor.authorLipový, Břetislavcs
dc.contributor.authorFaldyna, Martincs
dc.contributor.authorGöpfert, Eduardcs
dc.contributor.authorHoloubek, Jakubcs
dc.contributor.authorPavlovský, Zdeněkcs
dc.contributor.authorVícenová, Monikacs
dc.contributor.authorBlahnová, Veronikacs
dc.contributor.authorHearnden, Vanessacs
dc.contributor.authorFilova, Evacs
dc.coverage.issue6cs
dc.coverage.volume9cs
dc.date.issued2021-06-01cs
dc.description.abstractWound healing is a process regulated by a complex interaction of multiple growth factors including fibroblast growth factor 2 (FGF2). Although FGF2 appears in several tissue engineered studies, its applications are limited due to its low stability both in vitro and in vivo. Here, this shortcoming is overcome by a unique nine-point mutant of the low molecular weight isoform FGF2 retaining full biological activity even after twenty days at 37 degrees C. Crosslinked freeze-dried 3D porous collagen/chitosan scaffolds enriched with this hyper stable recombinant human protein named FGF2-STAB(R) were tested for in vitro biocompatibility and cytotoxicity using murine 3T3-A31 fibroblasts, for angiogenic potential using an ex ovo chick chorioallantoic membrane assay and for wound healing in vivo with 3-month old white New Zealand rabbits. Metabolic activity assays indicated the positive effect of FGF2-STAB(R) already at very low concentrations (0.01 mu g/mL). The angiogenic properties examined ex ovo showed enhanced vascularization of the tested scaffolds. Histological evaluation and gene expression analysis by RT-qPCR proved newly formed granulation tissue at the place of a previous skin defect without significant inflammation infiltration in vivo. This work highlights the safety and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB(R) protein. Moreover, these sponges could be used as scaffolds for growing cells for dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.en
dc.formattextcs
dc.format.extent1-27cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationBiomedicines. 2021, vol. 9, issue 6, p. 1-27.en
dc.identifier.doi10.3390/biomedicines9060590cs
dc.identifier.issn2227-9059cs
dc.identifier.orcid0000-0001-5281-7045cs
dc.identifier.orcid0000-0002-4694-298Xcs
dc.identifier.orcid0000-0003-1683-5144cs
dc.identifier.orcid0000-0002-4898-8041cs
dc.identifier.orcid0000-0002-5132-4094cs
dc.identifier.other171955cs
dc.identifier.researcheridD-7762-2012cs
dc.identifier.researcheridB-3167-2014cs
dc.identifier.scopus12039667200cs
dc.identifier.scopus56108279900cs
dc.identifier.urihttp://hdl.handle.net/11012/200493
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofBiomedicinescs
dc.relation.urihttps://www.mdpi.com/2227-9059/9/6/590cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2227-9059/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectcollagenen
dc.subjectchitosanen
dc.subjectscaffolden
dc.subjectFGF2en
dc.subjectskin regenerationen
dc.subjecttissue engineeringen
dc.titleHealing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluationen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-171955en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:51:00en
sync.item.modts2025.01.17 15:17:06en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé biomateriálycs
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