Accelular nanofbrous bilayer scafold intrapenetrated with polydopamine network and implemented into a full-thickness wound of a white-pig model afects infammation and healing process

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dc.contributor.authorKacvinská, Katarínacs
dc.contributor.authorPavliňáková, Veronikacs
dc.contributor.authorPoláček, Petrcs
dc.contributor.authorMichlovská, Lenkacs
dc.contributor.authorHefka Blahnová, Veronikacs
dc.contributor.authorFilová, Evacs
dc.contributor.authorKnoz, Martincs
dc.contributor.authorLipový, Břetislavcs
dc.contributor.authorHoloubek, Jakubcs
dc.contributor.authorFaldyna, Martincs
dc.contributor.authorPavlovský, Zdeněkcs
dc.contributor.authorVícenová, Monikacs
dc.contributor.authorCvanová, Michaelacs
dc.contributor.authorJarkovský, Jiřícs
dc.contributor.authorVojtová, Lucycs
dc.coverage.issue1cs
dc.coverage.volume21cs
dc.date.accessioned2023-07-21T06:53:48Z
dc.date.available2023-07-21T06:53:48Z
dc.date.issued2023-03-07cs
dc.description.abstractTreatment of complete loss of skin thickness requires expensive cellular materials and limited skin grafts used as temporary coverage. This paper presents an acellular bilayer scaffold modified with polydopamine (PDA), which is designed to mimic a missing dermis and a basement membrane (BM). The alternate dermis is made from freeze-dried collagen and chitosan (Coll/Chit) or collagen and a calcium salt of oxidized cellulose (Coll/CaOC). Alternate BM is made from electrospun gelatin (Gel), polycaprolactone (PCL), and CaOC. Morphological and mechanical analyzes have shown that PDA significantly improved the elasticity and strength of collagen microfibrils, which favorably affected swelling capacity and porosity. PDA significantly supported and maintained metabolic activity, proliferation, and viability of the murine fibroblast cell lines. The in vivo experiment carried out in a domestic Large white pig model resulted in the expression of pro-inflammatory cytokines in the first 1-2 weeks, giving the idea that PDA and/or CaOC trigger the early stages of inflammation. Otherwise, in later stages, PDA caused a reduction in inflammation with the expression of the anti-inflammatory molecule IL10 and the transforming growth factor beta (TGF beta 1), which could support the formation of fibroblasts. Similarities in treatment with native porcine skin suggested that the bilayer can be used as an implant for full-thickness skin wounds and thus eliminate the use of skin grafts.en
dc.formattextcs
dc.format.extent1-24cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJournal of Nanobiotechnology. 2023, vol. 21, issue 1, p. 1-24.en
dc.identifier.doi10.1186/s12951-023-01822-5cs
dc.identifier.issn1477-3155cs
dc.identifier.other183156cs
dc.identifier.urihttp://hdl.handle.net/11012/213580
dc.language.isoencs
dc.publisherBioMed Centralcs
dc.relation.ispartofJournal of Nanobiotechnologycs
dc.relation.urihttps://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-023-01822-5cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1477-3155/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectBilayeren
dc.subjectChitosanen
dc.subjectCollagenen
dc.subjectOxidized celluloseen
dc.subjectPolydopamineen
dc.subjectWound healingen
dc.titleAccelular nanofbrous bilayer scafold intrapenetrated with polydopamine network and implemented into a full-thickness wound of a white-pig model afects infammation and healing processen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-183156en
sync.item.dbtypeVAVen
sync.item.insts2023.07.21 08:53:48en
sync.item.modts2023.07.21 08:32:47en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé biomateriálycs
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