Accumulation of Poly(3-hydroxybutyrate) Helps Bacterial Cells to Survive Freezing

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dc.contributor.authorObruča, Stanislavcs
dc.contributor.authorSedláček, Petrcs
dc.contributor.authorMravec, Filipcs
dc.contributor.authorKučera, Dancs
dc.contributor.authorBenešová, Pavlacs
dc.contributor.authorMárová, Ivanacs
dc.coverage.issue6cs
dc.coverage.volume11cs
dc.date.accessioned2020-08-04T10:59:45Z
dc.date.available2020-08-04T10:59:45Z
dc.date.issued2016-06-17cs
dc.description.abstractAccumulation of polyhydroxybutyrate (PHB) seems to be a common metabolic strategy adopted by many bacteria to cope with cold environments. This work aimed at evaluating and understanding the cryoprotective effect of PHB. At first a monomer of PHB, 3-hydroxybutyrate, was identified as a potent cryoprotectant capable of protecting model enzyme (lipase), yeast (Saccharomyces cerevisiae) and bacterial cells (Cupriavidus necator) against the adverse effects of freezing-thawing cycles. Further, the viability of the frozen-thawed PHB accumulating strain of C. necator was compared to that of the PHB non-accumulating mutant. The presence of PHB granules in cells was revealed to be a significant advantage during freezing. This might be attributed to the higher intracellular level of 3-hydroxybutyrate in PHB accumulating cells (due to the action of parallel PHB synthesis and degradation, the so-called PHB cycle), but the cryoprotective effect of PHB granules seems to be more complex. Since intracellular PHB granules retain highly flexible properties even at extremely low temperatures (observed by cryo-SEM), it can be expected that PHB granules protect cells against injury from extracellular ice. Finally, thermal analysis indicates that PHB-containing cells exhibit a higher rate of transmembrane water transport, which protects cells against the formation of intracellular ice which usually has fatal consequences.en
dc.description.abstractAkumulace polyhydroxybutyrate (PHB) se zdá být společnou metabolickou strategií adoptovanou mnoha bakteriemi za účelem odolat chladnému prostředí. Cílem této práce bylo pochopení kryoprotektivního účinku PHB. Monomer PHB - 3-hydroxybutyrát, byl identifikován jako silný protektant schopný chránit enzymy, kvasinky (Saccharomyces cerevisiae) a bakteriální buňky (Cupriavidus necator) proti nepříznivým účinkům cyklů zmrazování rozmrazování.cs
dc.formattextcs
dc.format.extent1-16cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPLOS ONE. 2016, vol. 11, issue 6, p. 1-16.en
dc.identifier.doi10.1371/journal.pone.0157778cs
dc.identifier.issn1932-6203cs
dc.identifier.other126803cs
dc.identifier.urihttp://hdl.handle.net/11012/65334
dc.language.isoencs
dc.publisherPLoScs
dc.relation.ispartofPLOS ONEcs
dc.relation.urihttp://europepmc.org/articles/PMC4912086?pdf=rendercs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1932-6203/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectPolyhydroxybutyrát
dc.subject3-hydroxybutyrát
dc.subjectkryoprotektanty
dc.subjectvoda vnitrobuněčná
dc.subjectstresová odpověď
dc.subjectPolyhydroxybutyrateen
dc.subject3-hydroxybuyrateen
dc.subjectcryoprotectantsen
dc.subjectintracellular wateren
dc.subjectstress responseen
dc.titleAccumulation of Poly(3-hydroxybutyrate) Helps Bacterial Cells to Survive Freezingen
dc.title.alternativeAkumulace Poly(3-hydroxybutyrate) pomáhá bakteriálním buňkám přežít zmrazenícs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-126803en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 12:59:45en
sync.item.modts2020.08.04 12:42:04en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. CMV - laboratoř biokoloidůcs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. CMV - laboratoř biotechnologiícs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
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