Diversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791T

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dc.contributor.authorSedlář, Karelcs
dc.contributor.authorJakubíčková, Markétacs
dc.contributor.authorBezdíček, Matějcs
dc.contributor.authorBranská, Barboracs
dc.contributor.authorLengerová, Martinacs
dc.contributor.authorPatáková, Petracs
dc.contributor.authorVítková, Helenacs
dc.coverage.issue7cs
dc.coverage.volume9cs
dc.date.issued2021-07-10cs
dc.description.abstractClostridium beijerinckii is a relatively widely studied, yet non-model, bacterium. While 246 genome assemblies of its various strains are available currently, the diversity of the whole species has not been studied, and it has only been analyzed in part for a missing genome of the type strain. Here, we sequenced and assembled the complete genome of the type strain Clostridium beijerinckii DSM 791T, composed of a circular chromosome and a circular megaplasmid, and used it for a comparison with other genomes to evaluate diversity and capture the evolution of the whole species. We found that strains WB53 and HUN142 were misidentified and did not belong to the Clostridium beijerinckii species. Additionally, we filtered possibly misassembled genomes, and we used the remaining 237 high-quality genomes to define the pangenome of the whole species. By its functional annotation, we showed that the core genome contains genes responsible for basic metabolism, while the accessory genome has genes affecting final phenotype that may vary among different strains. We used the core genome to reconstruct the phylogeny of the species and showed its great diversity, which complicates the identification of particular strains, yet hides possibilities to reveal hitherto unreported phenotypic features and processes utilizable in biotechnology.en
dc.formattextcs
dc.format.extent1-16cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationProcesses. 2021, vol. 9, issue 7, p. 1-16.en
dc.identifier.doi10.3390/pr9071196cs
dc.identifier.issn2227-9717cs
dc.identifier.orcid0000-0002-8269-4020cs
dc.identifier.orcid0000-0002-0205-6935cs
dc.identifier.orcid0000-0003-4562-2746cs
dc.identifier.other171987cs
dc.identifier.researcheridK-1120-2014cs
dc.identifier.researcheridR-3181-2018cs
dc.identifier.researcheridD-5194-2014cs
dc.identifier.scopus56309904900cs
dc.identifier.scopus57202469794cs
dc.identifier.scopus36521691000cs
dc.identifier.urihttp://hdl.handle.net/11012/200480
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofProcessescs
dc.relation.urihttps://www.mdpi.com/2227-9717/9/7/1196cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2227-9717/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectbutanolen
dc.subjectABEen
dc.subjectIBEen
dc.subjectcore genomeen
dc.subjectaccessory genomeen
dc.subjectpan genomeen
dc.titleDiversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791Ten
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-171987en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:39:47en
sync.item.modts2025.01.17 18:33:47en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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