The Effect of Rhodamine-Derived Superparamagnetic Maghemite Nanoparticles on the Motility of Human Mesenchymal Stem Cells and Mouse Embryonic Fibroblast Cells

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dc.contributor.authorChmelíková, Larisacs
dc.contributor.authorSkopalík, Josefcs
dc.contributor.authorChmelík, Jiřícs
dc.contributor.authorZumberg, Innacs
dc.contributor.authorČmiel, Vratislavcs
dc.contributor.authorPoláková, Kateřinacs
dc.contributor.authorProvazník, Valentýnacs
dc.coverage.issue7cs
dc.coverage.volume24cs
dc.date.issued2019-03-27cs
dc.description.abstractNanoparticles have become popular in life sciences in the last few years. They have been produced in many variants and have recently been used in both biological experiments and in clinical applications. Due to concerns over nanomaterial risks, there has been a dramatic increase in investigations focused on safety research. The aim of this paper is to present the advanced testing of rhodamine-derived superparamagnetic maghemite nanoparticles (SAMN-R), which are used for their nontoxicity, biocompatibility, biodegradability, and magnetic properties. Recent results were expanded upon from the basic cytotoxic tests to evaluate cell proliferation and migration potential. Two cell types were used for the cell proliferation and tracking study: mouse embryonic fibroblast cells (3T3) and human mesenchymal stem cells (hMSCs). Advanced microscopic methods allowed for the precise quantification of the function of both cell types. This study has demonstrated that a dose of nanoparticles lower than 20 microg-cm -2 per area of the dish does not negatively affect the cells’ morphology, migration, cytoskeletal function, proliferation, potential for wound healing, and single-cell migration in comparison to standard CellTracker Green CMFDA (5-chloromethylfluorescein diacetate). A higher dose of nanoparticles could be a potential risk for cytoskeletal folding and detachment of the cells from the solid extracellular matrix.en
dc.formattextcs
dc.format.extent1192-1209cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMOLECULES. 2019, vol. 24, issue 7, p. 1192-1209.en
dc.identifier.doi10.3390/molecules24071192cs
dc.identifier.issn1420-3049cs
dc.identifier.orcid0000-0002-3178-4202cs
dc.identifier.orcid0000-0001-9950-6279cs
dc.identifier.orcid0000-0001-7250-1945cs
dc.identifier.orcid0000-0002-0441-9840cs
dc.identifier.orcid0000-0002-3422-7938cs
dc.identifier.other156464cs
dc.identifier.researcheridD-3886-2018cs
dc.identifier.researcheridH-9359-2017cs
dc.identifier.researcheridAAC-5812-2019cs
dc.identifier.researcheridF-4121-2012cs
dc.identifier.scopus57188881119cs
dc.identifier.scopus57188877911cs
dc.identifier.scopus54960991100cs
dc.identifier.scopus6701729526cs
dc.identifier.urihttp://hdl.handle.net/11012/179268
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMOLECULEScs
dc.relation.urihttps://www.mdpi.com/1420-3049/24/7/1192cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1420-3049/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectmagnetic nanoparticlesen
dc.subjectmesenchymal stem cellsen
dc.subjectfibroblast cellsen
dc.subjectcytotoxicityen
dc.subjectwound healing assayen
dc.subjectsingle-cell migrationen
dc.titleThe Effect of Rhodamine-Derived Superparamagnetic Maghemite Nanoparticles on the Motility of Human Mesenchymal Stem Cells and Mouse Embryonic Fibroblast Cellsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-156464en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:39:41en
sync.item.modts2025.01.17 15:35:34en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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