Automated alignment method for coherence-controlled holographic microscope

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dc.contributor.authorDostál, Zbyněkcs
dc.contributor.authorSlabý, Tomášcs
dc.contributor.authorKvasnica, Lukášcs
dc.contributor.authorLošťák, Martincs
dc.contributor.authorKřížová, Anetacs
dc.contributor.authorChmelík, Radimcs
dc.coverage.issue11cs
dc.coverage.volume20cs
dc.date.issued2015-10-28cs
dc.description.abstractCoherence-controlled holographic microscope (CCHM) was developed particularly for quantitative phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM in low-coherence mode extends DHM in the study of living cells. However, this advantage is compensated by sensitivity of the system to easily become misaligned, which is a serious hindrance to wanted performance. Therefore, it became clear that introduction of a self-correcting system is inevitable. Accordingly, we had to devise a theory of a suitable control and design an automated alignment system for CCHM. The modulus of the reconstructed holographic signal was identified as a significant variable for guiding the alignment procedures. From this, we derived the original basic realignment three-dimensional algorithm, which encompasses a unique set of procedures for automated alignment that contains processes for initial and advanced alignment as well as long-term maintenance of microscope tuning. All of these procedures were applied to a functioning microscope and the tested processes were successfully validated. Finally, in such a way, CCHM is enabled to substantially contribute to study of biology, particularly of cancer cells in vitro.en
dc.description.abstractV článku je popsaná automatická metoda ladění pro koherencí řízenou holografickou mikroskopii. Všechny popsané procedury byly aplikovány na fungující mikroskop a testované procesy byly úspěšně ověřeny.cs
dc.formattextcs
dc.format.extent1-8cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJOURNAL OF BIOMEDICAL OPTICS. 2015, vol. 20, issue 11, p. 1-8.en
dc.identifier.doi10.1117/1.JBO.20.11.111215cs
dc.identifier.issn1083-3668cs
dc.identifier.orcid0000-0002-1264-4781cs
dc.identifier.orcid0000-0002-9919-6204cs
dc.identifier.orcid0000-0001-5410-4794cs
dc.identifier.other117913cs
dc.identifier.researcheridE-2088-2012cs
dc.identifier.researcheridG-8475-2014cs
dc.identifier.researcheridD-7616-2012cs
dc.identifier.scopus6603192372cs
dc.identifier.urihttp://hdl.handle.net/11012/194979
dc.language.isoencs
dc.publisherSPIEcs
dc.relation.ispartofJOURNAL OF BIOMEDICAL OPTICScs
dc.relation.urihttps://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics/volume-20/issue-11/111215/Automated-alignment-method-for-coherence-controlled-holographic-microscope/10.1117/1.JBO.20.11.111215.fullcs
dc.rightsCreative Commons Attribution 3.0 Unportedcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1083-3668/cs
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/cs
dc.subjectholographic microscopyen
dc.subjectquantitative phase imagingen
dc.subjectautomated alignmenten
dc.subjectholographic signalen
dc.subjectholografická mikroskopie
dc.subjectkvantitativní fázové zobrazování
dc.subjectautomatizované ladění
dc.subjectholografický signál
dc.titleAutomated alignment method for coherence-controlled holographic microscopeen
dc.title.alternativeAutomatizovaná metoda ladění pro koherenci řízenou holografickou mikroskopiics
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-117913en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:41en
sync.item.modts2025.01.17 16:40:05en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Experimentální biofotonikacs
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