Urany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cells

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dc.contributor.authorMrázová, Kateřinacs
dc.contributor.authorBačovský, Jaromírcs
dc.contributor.authorKroupová, Zuzanacs
dc.contributor.authorSlaninová, Evacs
dc.contributor.authorObruča, Stanislavcs
dc.contributor.authorFritz, Inescs
dc.contributor.authorKrzyžánek, Vladislavcs
dc.coverage.issue4cs
dc.coverage.volume11cs
dc.date.issued2023-03-29cs
dc.description.abstractSample preparation protocols for conventional high voltage transmission electron microscopy (TEM) heavily rely on the usage of staining agents containing various heavy metals, most commonly uranyl acetate and lead citrate. However high toxicity, rising legal regulations, and problematic waste disposal of uranyl acetate have increased calls for the reduction or even complete replacement of this staining agent. One of the strategies for uranyless imaging is the employment of low-voltage transmission electron microscopy. To investigate the influence of different imaging and staining strategies on the final image of cyanobacterial cells, samples stained by uranyl acetate with lead citrate, as well as unstained samples, were observed using TEM and accelerating voltages of 200 kV or 25 kV. Moreover, to examine the possibilities of reducing chromatic aberration, which often causes issues when imaging using electrons of lower energies, samples were also imaged using a scanning transmission electron microscopy at 15 kV accelerating voltages. The results of this study demonstrate that low-voltage electron microscopy offers great potential for uranyless electron microscopy.en
dc.formattextcs
dc.format.extent1-13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMicroorganisms. 2023, vol. 11, issue 4, p. 1-13.en
dc.identifier.doi10.3390/microorganisms11040888cs
dc.identifier.issn2076-2607cs
dc.identifier.orcid0000-0002-8956-6515cs
dc.identifier.orcid0000-0002-1808-8632cs
dc.identifier.orcid0000-0002-9270-195Xcs
dc.identifier.other184203cs
dc.identifier.scopus25621797900cs
dc.identifier.urihttp://hdl.handle.net/11012/213621
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMicroorganismscs
dc.relation.urihttps://www.mdpi.com/2076-2607/11/4/888cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2076-2607/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectlow voltage electron microscopyen
dc.subjecturanyl acetateen
dc.subjectcontrasting agentsen
dc.subjecttransmission electron microscopyen
dc.subjectSynechocystisen
dc.subjectpolyhydroxyalkanoatesen
dc.titleUrany-Less Low Voltage Transmission Electron Microscopy: A Powerful Tool for Ultrastructural Studying of Cyanobacterial Cellsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-184203en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:38:42en
sync.item.modts2025.01.17 16:42:00en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
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