Immobilization of Platelet-Rich Plasma onto COOH Plasma-Coated PCL Nanofibers Boost Viability and Proliferation of Human Mesenchymal Stem Cells

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dc.contributor.authorSolovieva, Anastasiyacs
dc.contributor.authorMiroshnichenko, Svetlanacs
dc.contributor.authorKovalskii, Andreycs
dc.contributor.authorPermyakova, Elizavetacs
dc.contributor.authorPopov, Zakharcs
dc.contributor.authorDvořáková, Evacs
dc.contributor.authorKiryukhantsev-Korneev, Philipcs
dc.contributor.authorObrosov, Alekseics
dc.contributor.authorPolčák, Josefcs
dc.contributor.authorZajíčková, Lenkacs
dc.contributor.authorShtansky, Dmitry V.cs
dc.contributor.authorManakhov, Antoncs
dc.coverage.issue12cs
dc.coverage.volume9cs
dc.date.accessioned2020-08-04T11:04:36Z
dc.date.available2020-08-04T11:04:36Z
dc.date.issued2017-12-20cs
dc.description.abstractThe scaffolds made of polycaprolactone (PCL) are actively employed in different areas of biology and medicine, especially in tissue engineering. However, the usage of unmodified PCL is significantly restricted by the hydrophobicity of its surface, due to the fact that its inert surface hinders the adhesion of cells and the cell interactions on PCL surface. In this work, the surface of PCL nanofibers is modified by Ar/CO2/C2H4 plasma depositing active COOH groups in the amount of 0.57 at % that were later used for the immobilization of platelet-rich plasma (PRP). The modification of PCL nanofibers significantly enhances the viability and proliferation (by hundred times) of human mesenchymal stem cells, and decreases apoptotic cell death to a normal level. According to X-ray photoelectron spectroscopy (XPS), after immobilization of PRP, up to 10.7 at % of nitrogen was incorporated into the nanofibers surface confirming the grafting of proteins. Active proliferation and sustaining the cell viability on nanofibers with immobilized PRP led to an average number of cells of 258+-12.9 and 364+-34.5 for nanofibers with ionic and covalent bonding of PRP, respectively. Hence, our new method for the modification of PCL nanofibers with PRP opens new possibilities for its application in tissue engineering.en
dc.formattextcs
dc.format.extent736-1-736-14cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPolymers. 2017, vol. 9, issue 12, p. 736-1-736-14.en
dc.identifier.doi10.3390/polym9120736cs
dc.identifier.issn2073-4360cs
dc.identifier.other143271cs
dc.identifier.urihttp://hdl.handle.net/11012/137992
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofPolymerscs
dc.relation.urihttps://www.mdpi.com/2073-4360/9/12/736cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2073-4360/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectpolycaprolactoneen
dc.subjectnanofibersen
dc.subjectCOOH plasmaen
dc.subjectplatelet-rich plasmaen
dc.subjectcell viabilityen
dc.subjectPRP immobilizationen
dc.titleImmobilization of Platelet-Rich Plasma onto COOH Plasma-Coated PCL Nanofibers Boost Viability and Proliferation of Human Mesenchymal Stem Cellsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-143271en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:04:36en
sync.item.modts2020.08.04 12:45:07en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Příprava a charakterizace nanostrukturcs
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav fyzikálního inženýrstvícs
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Příprava a charakterizace nanostruktur
Ústav fyzikálního inženýrství