Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

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dc.contributor.authorKolek, Jancs
dc.contributor.authorSedlář, Karelcs
dc.contributor.authorProvazník, Valentýnacs
dc.contributor.authorPatáková, Petracs
dc.coverage.issue1cs
dc.coverage.volume9cs
dc.date.issued2016-01-20cs
dc.description.abstractButanol is currently one of the most discussed biofuels. Its use provides many benefits in comparison to bio-ethanol, but the price of its fermentative production is still high. Genetic improvements could help solve many problems associated with butanol production during ABE fermentation, such as its toxicity, low concentration achievable in the cultivation medium, the need for a relatively expensive substrate, and many more. Clostridium pasteurianum NRRL B-598 is non-type strain producing butanol, acetone, and a negligible amount of ethanol. Its main benefits are high oxygen tolerance, utilization of a wide range of carbon and nitrogen sources, and the availability of its whole genome sequence. However, there is no established method for the transfer of foreign DNA into this strain; this is the next step necessary for progress in its use for butanol production.en
dc.formattextcs
dc.format.extent1-14cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationBIOTECHNOL BIOFUELS. 2016, vol. 9, issue 1, p. 1-14.en
dc.identifier.doi10.1186/s13068-016-0436-ycs
dc.identifier.issn1754-6834cs
dc.identifier.orcid0000-0002-8269-4020cs
dc.identifier.orcid0000-0002-3422-7938cs
dc.identifier.other120980cs
dc.identifier.researcheridK-1120-2014cs
dc.identifier.researcheridF-4121-2012cs
dc.identifier.scopus56309904900cs
dc.identifier.scopus6701729526cs
dc.identifier.urihttp://hdl.handle.net/11012/69198
dc.language.isoencs
dc.publisherBioMed Centralcs
dc.relation.ispartofBIOTECHNOL BIOFUELScs
dc.relation.urihttp://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0436-ycs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1754-6834/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectClostridiumen
dc.subjectButanolen
dc.subjectTransformationen
dc.subjectConjugationen
dc.subjectMethylationen
dc.subjectDamen
dc.subjectDcmen
dc.subjectElectroporationen
dc.subjectSonoporationen
dc.titleDam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporationen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-120980en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:40:04en
sync.item.modts2025.01.17 16:33:37en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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